{"data":{"id":"10.6084/m9.figshare.1568816","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568816","prefix":"10.6084","suffix":"m9.figshare.1568816","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yang-Kolodji, Gloria","nameType":"Personal","givenName":"Gloria","familyName":"Yang-Kolodji","affiliation":[],"nameIdentifiers":[]},{"name":"Mumenthaler, Shannon M.","nameType":"Personal","givenName":"Shannon M.","familyName":"Mumenthaler","affiliation":[],"nameIdentifiers":[]},{"name":"Mehta, Arjun","nameType":"Personal","givenName":"Arjun","familyName":"Mehta","affiliation":[],"nameIdentifiers":[]},{"name":"Lingyun Ji","affiliation":[],"nameIdentifiers":[]},{"name":"Debu Tripathy","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Phosphorylated ribosomal S6 (p-rpS6) as a post-treatment indicator of HER2 signalling targeted drug resistance"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Pharmacology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/1354750x.2015.1068865","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["392202 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eObjective\u003c/i\u003e: To identify clinically relevant predictive biomarkers of trastuzumab resistance.\u003ci\u003eMaterial and methods\u003c/i\u003e: MTT, FACS assays, immunoblotting and immunocytochemistry were used to phenotypically characterize drug responses of two cell models BT474R and SKBR3R. Student's \u003ci\u003et\u003c/i\u003e-test and Spearman's correlation were applied for statistic analysis.\u003ci\u003eResults\u003c/i\u003e: The activity of a downstream effector of the HER2 pathway phosphorylated ribosomal protein S6 (p-rpS6), was suppressed by trastuzumab in the parental cell lines yet remained unchanged in the resistant cells following treatment. The level of p-rpS6 was inversely correlated to the drug induced growth inhibition of trastuzumab-resistant cells when they are treated with selected HER2 targeting drugs.\u003ci\u003eConclusion\u003c/i\u003e: p-rpS6 is a robust post-treatment indicator of HER2 pathway-targeted therapy resistance.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Phosphorylated_ribosomal_S6_p_rpS6_as_a_post_treatment_indicator_of_HER2_signalling_targeted_drug_resistance/1568816","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"s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