{"data":{"id":"10.6084/m9.figshare.1568771","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568771","prefix":"10.6084","suffix":"m9.figshare.1568771","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yan, Quan","givenName":"Quan","familyName":"Yan","affiliation":[],"nameIdentifiers":[]},{"name":"Zhu, Hong","givenName":"Hong","familyName":"Zhu","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Feng-hua","givenName":"Feng-hua","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Feng, Jing-yang","givenName":"Jing-yang","familyName":"Feng","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Wen-qiu","givenName":"Wen-qiu","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Shi, Xiang","givenName":"Xiang","familyName":"Shi","affiliation":[],"nameIdentifiers":[]},{"name":"Zhou, Yan-ping","givenName":"Yan-ping","familyName":"Zhou","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Xi","givenName":"Xi","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Sun, Xiao-dong","givenName":"Xiao-dong","familyName":"Sun","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Inhibition of TRB3 Protects Photoreceptors against Endoplasmic Reticulum Stress-Induced Apoptosis after Experimental Retinal Detachment"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Neuroscience"},{"subject":"Physiology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"}],"contributors":[],"dates":[{"date":"2016-02-02","dateType":"Created"},{"date":"2023-06-01","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/02713683.2015.1006371","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1012767 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003ePurpose\u003c/i\u003e: To investigate the expression of tribbles homologue 3 (TRB3) and its regulation on endoplasmic reticulum stress (ERS)-induced photoreceptor apoptosis after retinal detachment (RD) using a rat model. \u003ci\u003eMethods\u003c/i\u003e: RD animal model was created in Wistar rats by subretinal injection of 1% sodium hyaluronate. At various time points after RD, expression of TRB3 was detected by quantitative real-time PCR and Western blotting. TRB3 protein distribution in retina was evaluated by immunohistochemistry. RNA interference was used to inhibit TRB3 expression and subretinal injection of lentivirus TRB3 shRNA (LV-TRB3-sh) was performed. The rats were then randomly divided into four groups: normal control group, RD group, vehicle + RD group and LV-TRB3-sh + RD group. The mRNA and protein level of TRB3 as well as Caspase-12 were detected. TdT-mediated fluorescein-16-dUTP nick-end labeling (TUNEL) assay was used to detect the apoptosis of retinal cells. Retinal outer nuclear layer (ONL) thickness was measured to assess retina damage in each group. \u003ci\u003eResults\u003c/i\u003e: TRB3 expression and TRB3-positive cell count were significantly increased after RD and peaked at day 3 after RD. The ratio of TUNEL-positive photoreceptors and expression of ERS-induced apoptosis marker Caspase-12 in LV-TRB3-sh + RD group were significantly reduced. The ONL thickness in LV-TRB3-sh + RD group was thicker than that both in RD group and vehicle + RD group. \u003ci\u003eConclusion\u003c/i\u003e: TRB3 expression is up-regulated in retinas after RD and knockdown of TRB3 protects photoreceptors against ERS-induced apoptosis. TRB3 may be a crucial molecule in photoreceptor apoptosis induced by ERS after RD.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Inhibition_of_TRB3_Protects_Photoreceptors_against_Endoplasmic_Reticulum_Stress_Induced_Apoptosis_after_Experimental_Retinal_Detachment/1568771","contentUrl":null,"metadataVersion":5,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":1,"citationsOverTime":[{"year":"2024","total":1}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-10-08T11:43:41.000Z","registered":"2015-10-08T11:43:42.000Z","published":"2016","updated":"2024-03-24T12:20:28.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1568771","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.3109/02713683.2015.1006371","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}