{"data":{"id":"10.6084/m9.figshare.1568746","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568746","prefix":"10.6084","suffix":"m9.figshare.1568746","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Lee, Wen-Chi","nameType":"Personal","givenName":"Wen-Chi","familyName":"Lee","affiliation":[],"nameIdentifiers":[]},{"name":"Shin-Hua Lu","affiliation":[],"nameIdentifiers":[]},{"name":"Ming-Hsuan Lu","affiliation":[],"nameIdentifiers":[]},{"name":"Chen-Jui Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Shu-Hsing Wu","affiliation":[],"nameIdentifiers":[]},{"name":"Ho-Ming Chen","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Asymmetric bulges and mismatches determine 20-nt microRNA formation in plants"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Evolutionary Biology"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1079682","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["426115 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Plant microRNAs (miRNAs) are predominantly 21 nucleotides (nt) long but non-canonical lengths of 22 and 20 nt are commonly observed in diverse plant species. While miRNAs longer than 21 nt can be attributed to the neglect of unpaired bases within asymmetric bulges by the ruler function of DICER-LIKE 1 (DCL1), how 20-nt miRNA is generated remains obscure. Analysis of small RNA data revealed that 20-nt miRNA can be divided into 3 main groups featured by atypical 3′ overhangs or shorter duplex regions. Asymmetric bulges or mismatches at specific positions are commonly observed within each group and were shown to be crucial for 20-nt miRNA formation. Analysis of DCL1 cleavage sites on 20-nt miRNA precursors suggests that these determinants might alter precursor structure or trigger 3′-end decay of mature miRNA. The results herein advance our understanding of miRNA biogenesis and demonstrate that the effect of asymmetric bulges on miRNA length could be position-dependent.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjE1Njg3NDY8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5XZW4tQ2hpIExlZTwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlNoaW4tSHVhIEx1PC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+TWluZy1Ic3VhbiBMdTwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkNoZW4tSnVpIFlhbmc8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5TaHUtSHNpbmcgV3U8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5Iby1NaW5nIENoZW48L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogIDwvY3JlYXRvcnM+CiAgPHRpdGxlcz4KICAgIDx0aXRsZT5Bc3ltbWV0cmljIGJ1bGdlcyBhbmQgbWlzbWF0Y2hlcyBkZXRlcm1pbmUgMjAtbnQgbWljcm9STkEgZm9ybWF0aW9uIGluIHBsYW50czwvdGl0bGU+CiAgPC90aXRsZXM+CiAgPGRlc2NyaXB0aW9ucz4KICAgIDxkZXNjcmlwdGlvbiBkZXNjcmlwdGlvblR5cGU9IkFic3RyYWN0Ij4mbHQ7ZGl2Jmd0OyZsdDtwJmd0O1BsYW50IG1pY3JvUk5BcyAobWlSTkFzKSBhcmUgcHJlZG9taW5hbnRseSAyMSBudWNsZW90aWRlcyAobnQpIGxvbmcgYnV0IG5vbi1jYW5vbmljYWwgbGVuZ3RocyBvZiAyMiBhbmQgMjDCoG50IGFyZSBjb21tb25seSBvYnNlcnZlZCBpbiBkaXZlcnNlIHBsYW50IHNwZWNpZXMuIFdoaWxlIG1pUk5BcyBsb25nZXIgdGhhbiAyMcKgbnQgY2FuIGJlIGF0dHJpYnV0ZWQgdG8gdGhlIG5lZ2xlY3Qgb2YgdW5wYWlyZWQgYmFzZXMgd2l0aGluIGFzeW1tZXRyaWMgYnVsZ2VzIGJ5IHRoZSBydWxlciBmdW5jdGlvbiBvZiBESUNFUi1MSUtFIDEgKERDTDEpLCBob3cgMjAtbnQgbWlSTkEgaXMgZ2VuZXJhdGVkIHJlbWFpbnMgb2JzY3VyZS4gQW5hbHlzaXMgb2Ygc21hbGwgUk5BIGRhdGEgcmV2ZWFsZWQgdGhhdCAyMC1udCBtaVJOQSBjYW4gYmUgZGl2aWRlZCBpbnRvIDMgbWFpbiBncm91cHMgZmVhdHVyZWQgYnkgYXR5cGljYWwgM+KAsiBvdmVyaGFuZ3Mgb3Igc2hvcnRlciBkdXBsZXggcmVnaW9ucy4gQXN5bW1ldHJpYyBidWxnZXMgb3IgbWlzbWF0Y2hlcyBhdCBzcGVjaWZpYyBwb3NpdGlvbnMgYXJlIGNvbW1vbmx5IG9ic2VydmVkIHdpdGhpbiBlYWNoIGdyb3VwIGFuZCB3ZXJlIHNob3duIHRvIGJlIGNydWNpYWwgZm9yIDIwLW50IG1pUk5BIGZvcm1hdGlvbi4gQW5hbHlzaXMgb2YgRENMMSBjbGVhdmFnZSBzaXRlcyBvbiAyMC1udCBtaVJOQSBwcmVjdXJzb3JzIHN1Z2dlc3RzIHRoYXQgdGhlc2UgZGV0ZXJtaW5hbnRzIG1pZ2h0IGFsdGVyIHByZWN1cnNvciBzdHJ1Y3R1cmUgb3IgdHJpZ2dlciAz4oCyLWVuZCBkZWNheSBvZiBtYXR1cmUgbWlSTkEuIFRoZSByZXN1bHRzIGhlcmVpbiBhZHZhbmNlIG91ciB1bmRlcnN0YW5kaW5nIG9mIG1pUk5BIGJpb2dlbmVzaXMgYW5kIGRlbW9uc3RyYXRlIHRoYXQgdGhlIGVmZmVjdCBvZiBhc3ltbWV0cmljIGJ1bGdlcyBvbiBtaVJOQSBsZW5ndGggY291bGQgYmUgcG9zaXRpb24tZGVwZW5kZW50LiZsdDsvcCZndDsmbHQ7L2RpdiZndDs8L2Rlc2NyaXB0aW9uPgogIDwvZGVzY3JpcHRpb25zPgogIDxzdWJqZWN0cz4KICAgIDxzdWJqZWN0IHNjaGVtZVVSST0iaHR0cDovL3d3dy5hYnMuZ292LmF1L2F1c3N0YXRzL2Fic0AubnNmLzAvNkJCNDI3QUI5Njk2QzIyNUNBMjU3NDE4MDAwNDQ2M0UiIHN1YmplY3RTY2hlbWU9IkZPUiI+NjA1MDYgVmlyb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzY2hlbWVVUkk9Imh0dHA6Ly93d3cuYWJzLmdvdi5hdS9hdXNzdGF0cy9hYnNALm5zZi8wLzZCQjQyN0FCOTY5NkMyMjVDQTI1NzQxODAwMDQ0NjNFIiBzdWJqZWN0U2NoZW1lPSJGT1IiPjExMDMwOSBJbmZlY3Rpb3VzIERpc2Vhc2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RGV2ZWxvcG1lbnRhbCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvbG9naWNhbCBTY2llbmNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkNoZW1pc3RyeTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkV2b2x1dGlvbmFyeSBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvdGVjaG5vbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0Pk1vbGVjdWxhciBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+R2VuZXRpY3M8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DZWxsIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5NaWNyb2Jpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9jaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9waHlzaWNzPC9zdWJqZWN0PgogIDwvc3ViamVjdHM+CiAgPHB1Ymxpc2hlcj5UYXlsb3IgJmFtcDsgRnJhbmNpczwvcHVibGlzaGVyPgogIDxwdWJsaWNhdGlvblllYXI+MjAxNTwvcHVibGljYXRpb25ZZWFyPgogIDxkYXRlcz4KICAgIDxkYXRlIGRhdGVUeXBlPSJDcmVhdGVkIj4yMDE1LTEwLTA4PC9kYXRlPgogICAgPGRhdGUgZGF0ZVR5cGU9IlVwZGF0ZWQiPjIwMTUtMTItMDM8L2RhdGU+CiAgPC9kYXRlcz4KICA8cmVzb3VyY2VUeXBlIHJlc291cmNlVHlwZUdlbmVyYWw9IkRhdGFzZXQiPkRhdGFzZXQ8L3Jlc291cmNlVHlwZT4KICA8c2l6ZXM+CiAgICA8c2l6ZT40MjYxMTUgQnl0ZXM8L3NpemU+CiAgPC9zaXplcz4KICA8cmVsYXRlZElkZW50aWZpZXJzPgogICAgPHJlbGF0ZWRJZGVudGlmaWVyIHJlbGF0ZWRJZGVudGlmaWVyVHlwZT0iRE9JIiByZWxhdGlvblR5cGU9IklzU3VwcGxlbWVudFRvIj4xMC4xMDgwLzE1NDc2Mjg2LjIwMTUuMTA3OTY4MjwvcmVsYXRlZElkZW50aWZpZXI+CiAgPC9yZWxhdGVkSWRlbnRpZmllcnM+CiAgPHJpZ2h0c0xpc3Q+CiAgICA8cmlnaHRzIHJpZ2h0c1VSST0iaHR0cHM6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LzQuMC8iPkNDIEJZIDQuMDwvcmlnaHRzPgogIDwvcmlnaHRzTGlzdD4KPC9yZXNvdXJjZT4=","url":"https://tandf.figshare.com/articles/dataset/Asymmetric_bulges_and_mismatches_determine_20_nt_microRNA_formation_in_plants/1568746","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOver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