{"data":{"id":"10.6084/m9.figshare.1568257","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568257","prefix":"10.6084","suffix":"m9.figshare.1568257","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Holderbaum, Daniel Ferreira","nameType":"Personal","givenName":"Daniel Ferreira","familyName":"Holderbaum","affiliation":[],"nameIdentifiers":[]},{"name":"Cuhra, Marek","nameType":"Personal","givenName":"Marek","familyName":"Cuhra","affiliation":[],"nameIdentifiers":[]},{"name":"Wickson, Fern","nameType":"Personal","givenName":"Fern","familyName":"Wickson","affiliation":[],"nameIdentifiers":[]},{"name":"Orth, Afonso Inácio","nameType":"Personal","givenName":"Afonso Inácio","familyName":"Orth","affiliation":[],"nameIdentifiers":[]},{"name":"Nodari, Rubens Onofre","nameType":"Personal","givenName":"Rubens Onofre","familyName":"Nodari","affiliation":[],"nameIdentifiers":[]},{"name":"Bøhn, Thomas","nameType":"Personal","givenName":"Thomas","familyName":"Bøhn","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Chronic Responses of \u003ci\u003eDaphnia magna\u003c/i\u003e Under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Ecology"},{"subject":"Biotechnology"},{"subject":"Microbiology"},{"subject":"Medicine"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15287394.2015.1037877","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["61438 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Insect resistance is the second most common trait globally in cultivated genetically modified (GM) plants. Resistance is usually obtained by introducing into the plant’s genome genes from the bacterium \u003ci\u003eBacillus thuringiensis\u003c/i\u003e (Bt) coding for insecticidal proteins (Cry proteins or toxins) that target insect pests. The aim of this study was to examine the hypothesis that a chronic, high-dose dietary exposure to leaves of a Bt–maize hybrid (GM event MON810, expressing a transgenic or recombinant Cry1Ab toxin), exerted no adverse effects on fitness parameters of the aquatic nontarget organism \u003ci\u003eDaphnia magna\u003c/i\u003e (water flea) when compared to an identical control diet based on leaves of the non-GM near-isoline. Cry1Ab was immunologically detected and quantified in GM maize leaf material used for \u003ci\u003eDaphnia\u003c/i\u003e feed. A 69-kD protein near Bt’s active core-toxin size and a 34-kD protein were identified. The \u003ci\u003eD. magna\u003c/i\u003e bioassay showed a resource allocation to production of resting eggs and early fecundity in \u003ci\u003eD. magna\u003c/i\u003e fed GM maize, with adverse effects for body size and fecundity later in life. This is the first study to examine GM-plant leaf material in the \u003ci\u003eD. magna\u003c/i\u003e model, and provides of negative fitness effects of a MON810 maize hybrid in a nontarget model organism under chronic, high dietary exposure. Based upon these results, it is postulated that the observed transgenic proteins exert a nontarget effect in \u003ci\u003eD. magna\u003c/i\u003e and/or unintended changes were produced in the maize genome/metabolome by the transformation process, producing a nutritional difference between GM-maize and non-GM near-isoline.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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