{"data":{"id":"10.6084/m9.figshare.1568218.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568218.v1","prefix":"10.6084","suffix":"m9.figshare.1568218.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Prieto, Patricia","nameType":"Personal","givenName":"Patricia","familyName":"Prieto","affiliation":[],"nameIdentifiers":[]},{"name":"Rosales-Mendoza, César Eduardo","nameType":"Personal","givenName":"César Eduardo","familyName":"Rosales-Mendoza","affiliation":[],"nameIdentifiers":[]},{"name":"Terrón, Verónica","nameType":"Personal","givenName":"Verónica","familyName":"Terrón","affiliation":[],"nameIdentifiers":[]},{"name":"Toledano, Víctor","nameType":"Personal","givenName":"Víctor","familyName":"Toledano","affiliation":[],"nameIdentifiers":[]},{"name":"Cuadrado, Antonio","nameType":"Personal","givenName":"Antonio","familyName":"Cuadrado","affiliation":[],"nameIdentifiers":[]},{"name":"López-Collazo, Eduardo","nameType":"Personal","givenName":"Eduardo","familyName":"López-Collazo","affiliation":[],"nameIdentifiers":[]},{"name":"Bannenberg, Gerard","nameType":"Personal","givenName":"Gerard","familyName":"Bannenberg","affiliation":[],"nameIdentifiers":[]},{"name":"Martín-Sanz, Paloma","nameType":"Personal","givenName":"Paloma","familyName":"Martín-Sanz","affiliation":[],"nameIdentifiers":[]},{"name":"Fernández-Velasco, María","nameType":"Personal","givenName":"María","familyName":"Fernández-Velasco","affiliation":[],"nameIdentifiers":[]},{"name":"Boscá, Lisardo","nameType":"Personal","givenName":"Lisardo","familyName":"Boscá","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Activation of autophagy in macrophages by pro-resolving lipid mediators"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Meta Science"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"},{"subject":"Pharmacology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-03","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15548627.2015.1078958","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1568218","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["817758 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The resolution of inflammation is an active process driven by specialized pro-resolving lipid mediators, such as 15-epi-LXA\u003csub\u003e4\u003c/sub\u003e and resolvin D1 (RvD1), that promote tissue regeneration. Macrophages regulate the innate immune response being key players during the resolution phase to avoid chronic inflammatory pathologies. Their half-life is tightly regulated to accomplish its phagocytic function, allowing the complete cleaning of the affected area. The balance between apoptosis and autophagy appears to be essential to control the survival of these immune cells within the inflammatory context. In the present work, we demonstrate that 15-epi-LXA\u003csub\u003e4\u003c/sub\u003e and RvD1 at nanomolar concentrations promote autophagy in murine and human macrophages. Both compounds induced the MAP1LC3-I to MAP1LC3-II processing and the degradation of SQSTM1 as well as the formation of MAP1LC3\u003csup\u003e+\u003c/sup\u003e autophagosomes, a typical signature of autophagy. Furthermore, 15-epi-LXA\u003csub\u003e4\u003c/sub\u003e and RvD1 treatment favored the fusion of the autophagosomes with lysosomes, allowing the final processing of the autophagic vesicles. This autophagic response involves the activation of MAPK1 and NFE2L2 pathways, but by an MTOR-independent mechanism. Moreover, these pro-resolving lipids improved the phagocytic activity of macrophages \u003ci\u003evia\u003c/i\u003e NFE2L2. Therefore, 15-epi-LXA\u003csub\u003e4\u003c/sub\u003e and RvD1 improved both survival and functionality of macrophages, which likely supports the recovery of tissue homeostasis and avoiding chronic inflammatory diseases.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Activation_of_autophagy_in_macrophages_by_pro_resolving_lipid_mediators/1568218/1","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-20T06:19:56.000Z","registered":"2016-01-20T06:19:57.000Z","published":"2015","updated":"2020-09-04T15:28:54.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1568218.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}