{"data":{"id":"10.6084/m9.figshare.1568112","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568112","prefix":"10.6084","suffix":"m9.figshare.1568112","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Terashima, Minoru","nameType":"Personal","givenName":"Minoru","familyName":"Terashima","affiliation":[],"nameIdentifiers":[]},{"name":"Barbour, Samantha","nameType":"Personal","givenName":"Samantha","familyName":"Barbour","affiliation":[],"nameIdentifiers":[]},{"name":"Jianke Ren","affiliation":[],"nameIdentifiers":[]},{"name":"Weishi Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Yixing Han","affiliation":[],"nameIdentifiers":[]},{"name":"Muegge, Kathrin","nameType":"Personal","givenName":"Kathrin","familyName":"Muegge","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Effect of high fat diet on paternal sperm histone distribution and male offspring liver gene expression"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2016-03-11","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15592294.2015.1075691","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2866245 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Several studies have described phenotypic changes in the offspring of mice exposed to a variety of environmental factors, including diet, toxins, and stress; however, the molecular pathways involved in these changes remain unclear. Using a high fat diet (HFD)-induced obesity mouse model, we examined liver gene expression in male offspring and analyzed chromatin of paternal spermatozoa. We found that the hepatic mRNA level of 7 genes (out of 20 evaluated) was significantly altered in HFD male offspring compared to control mice, suggesting that phenotypic changes in the offspring depend on parental diet. We examined 7 imprinted loci in spermatozoa DNA from HFD-treated and control fathers by bisulfite sequencing, but did not detect changes in DNA methylation associated with HFD. Using chromatin immunoprecipitation followed by high-throughput sequencing, we found differential histone H3-occupancy at genes involved in the regulation of embryogenesis and differential H3K4me1-enrichment at transcription regulatory genes in HFD fathers vs. control mice. These results suggest that dietary exposure can modulate histone composition at regulatory genes implicated in developmental processes.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Effect_of_high_fat_diet_on_paternal_sperm_histone_distribution_and_male_offspring_liver_gene_expression/1568112/3","contentUrl":null,"metadataVersion":6,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-10-08T08:12:10.000Z","registered":"2015-10-08T08:12:11.000Z","published":"2015","updated":"2020-09-04T15:31:16.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1568112","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}