{"data":{"id":"10.6084/m9.figshare.1512834","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1512834","prefix":"10.6084","suffix":"m9.figshare.1512834","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Rajath Elias Soans","affiliation":[],"nameIdentifiers":[]},{"name":"Lim, Diane C","nameType":"Personal","givenName":"Diane C","familyName":"Lim","affiliation":[],"nameIdentifiers":[]},{"name":"Keenan, Brendan T","nameType":"Personal","givenName":"Brendan T","familyName":"Keenan","affiliation":[],"nameIdentifiers":[]},{"name":"Pack, Allan I","nameType":"Personal","givenName":"Allan I","familyName":"Pack","affiliation":[],"nameIdentifiers":[]},{"name":"Shackleford, James","nameType":"Personal","givenName":"James","familyName":"Shackleford","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Localized Protein Quantification of Blood Brain Barrier Vasculature in Brightfield IHC Images"}],"publisher":"figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Artificial Intelligence and Image Processing"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Computer and information sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"60102 Bioinformatics","subjectScheme":"FOR"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2015-08-16","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["77211183419 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"In this paper, we present an objective method for locally quantifying proteins in blood brain barrier (BBB) vasculature using standard immunohistochemistry (IHC) techniques and bright-field microscopy. Images from the hippocampal region at the BBB are acquired using bright-field microscopy and subjected to our immunohistochemistry quantification (IQ) algorithm which is designed to automatically identify and segment microvessels containing the protein glucose transporter 1 (GLUT1). Gabor filtering and k-means clustering are employed to isolate potential vascular structures within cryopsectioned slabs of the hippocampus, which are subsequently subjected to feature extraction followed by classification via decision forest. The false positive rate (FPR) of microvessel classification is characterized using synthetic and non-synthetic IHC image data for image entropies ranging between 3 and 8 bits. The average FPR for synthetic and non-synthetic IHC image data was found to be 5.48% and 5.04%, respectively.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/Localized_Protein_Quantification_of_Blood_Brain_Barrier_Vasculature_in_Brightfield_IHC_Images/1512834","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-08-18T23:45:37.000Z","registered":"2015-08-18T23:45:38.000Z","published":"2015","updated":"2020-09-04T16:04:13.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1512834","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}