{"data":{"id":"10.6084/m9.figshare.1501126","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1501126","prefix":"10.6084","suffix":"m9.figshare.1501126","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yueh Cho","affiliation":[],"nameIdentifiers":[]},{"name":"Yu, Chao-Yuan","nameType":"Personal","givenName":"Chao-Yuan","familyName":"Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Iwasa, Tatsuo","nameType":"Personal","givenName":"Tatsuo","familyName":"Iwasa","affiliation":[],"nameIdentifiers":[]},{"name":"Kanehara, Kazue","nameType":"Personal","givenName":"Kazue","familyName":"Kanehara","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Heterotrimeric G protein subunits differentially respond to endoplasmic reticulum stress in Arabidopsis"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Evolutionary Biology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2015-08-03","dateType":"Created"},{"date":"2018-08-31","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15592324.2015.1061162","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["321575 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Canonical heterotrimeric G proteins in eukaryotes are major components that localize at plasma membrane and transmit extracellular stimuli into the cell. Genome of a seed plant \u003ci\u003eArabidopsis thaliana\u003c/i\u003e encodes at least one Gα (\u003ci\u003eGPA1\u003c/i\u003e), one Gβ (\u003ci\u003eAGB1\u003c/i\u003e), and 3 Gγ (\u003ci\u003eAGG1, AGG2\u003c/i\u003e and \u003ci\u003eAGG3\u003c/i\u003e) subunits. The loss-of-function mutations of G protein subunit(s) cause multiple defects in development as well as biotic and abiotic stress responses. However, it remains elusive how these subunits differentially express these defects. Here, we report that Arabidopsis heterotrimeric G protein subunits differentially respond to the endoplasmic reticulum (ER) stress. An isolated homozygous mutant of \u003ci\u003eAGB1, agb1-3\u003c/i\u003e, was more sensitive to the tunicamycin-induced ER stress compared to the wild type and the other loss-of-function mutants of G protein subunits. Moreover, ER stress responsive genes were highly expressed in the \u003ci\u003eagb1-3\u003c/i\u003e plant. Our results indicate that \u003ci\u003eAGB1\u003c/i\u003e positively contributes to ER stress tolerance in Arabidopsis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Heterotrimeric_G_protein_subunits_differentially_respond_to_endoplasmic_reticulum_stress_in_Arabidopsis/1501126/3","contentUrl":null,"metadataVersion":8,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"part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