{"data":{"id":"10.6084/m9.figshare.1483479.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1483479.v1","prefix":"10.6084","suffix":"m9.figshare.1483479.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Sievers, Susanne","nameType":"Personal","givenName":"Susanne","familyName":"Sievers","affiliation":[],"nameIdentifiers":[]},{"name":"Lund, Anja","nameType":"Personal","givenName":"Anja","familyName":"Lund","affiliation":[],"nameIdentifiers":[]},{"name":"Menendez-Gil, Pilar","nameType":"Personal","givenName":"Pilar","familyName":"Menendez-Gil","affiliation":[],"nameIdentifiers":[]},{"name":"Aaraby Nielsen","affiliation":[],"nameIdentifiers":[]},{"name":"Mollerup, Maria Storm","nameType":"Personal","givenName":"Maria Storm","familyName":"Mollerup","affiliation":[],"nameIdentifiers":[]},{"name":"Nielsen, Stine Lambert","nameType":"Personal","givenName":"Stine Lambert","familyName":"Nielsen","affiliation":[],"nameIdentifiers":[]},{"name":"Larsson, Pernille Buch","nameType":"Personal","givenName":"Pernille Buch","familyName":"Larsson","affiliation":[],"nameIdentifiers":[]},{"name":"Borch-Jensen, Jonas","nameType":"Personal","givenName":"Jonas","familyName":"Borch-Jensen","affiliation":[],"nameIdentifiers":[]},{"name":"Johansson, Jörgen","nameType":"Personal","givenName":"Jörgen","familyName":"Johansson","affiliation":[],"nameIdentifiers":[]},{"name":"Kallipolitis, Birgitte Haahr","nameType":"Personal","givenName":"Birgitte Haahr","familyName":"Kallipolitis","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The multicopy sRNA LhrC controls expression of the oligopeptide-binding protein OppA in \u003ci\u003eListeria monocytogenes\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-09-02","dateType":"Created"},{"date":"2016-01-20","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1071011","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1483479","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["40767684 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eListeria monocytogenes\u003c/i\u003e is the causative agent of the foodborne disease listeriosis. During infection, \u003ci\u003eL. monocytogenes\u003c/i\u003e produces an array of non-coding RNAs, including the multicopy sRNA LhrC. These five, nearly identical sRNAs are highly induced in response to cell envelope stress and target the virulence adhesin \u003ci\u003elapB\u003c/i\u003e at the post-transcriptional level. Here, we demonstrate that LhrC controls expression of additional genes encoding cell envelope-associated proteins with virulence function. Using transcriptomics and proteomics, we identified a set of genes affected by LhrC in response to cell envelope stress. Three targets were significantly down-regulated by LhrC at both the RNA and protein level: \u003ci\u003elmo2349, tcsA\u003c/i\u003e and \u003ci\u003eoppA\u003c/i\u003e. All three genes encode membrane-associated proteins: A putative substrate binding protein of an amino acid ABC transporter (Lmo2349); the CD4+ T cell-stimulating antigen TcsA, and the oligopeptide binding protein OppA, of which the latter two are required for full virulence of \u003ci\u003eL. monocytogenes\u003c/i\u003e. For OppA, we show that LhrC acts by direct base paring to the ribosome binding site of the \u003ci\u003eoppA\u003c/i\u003e mRNA, leading to an impediment of its translation and a decreased mRNA level. The sRNA-mRNA interaction depends on two of three CU-rich regions in LhrC allowing binding of two \u003ci\u003eoppA\u003c/i\u003e mRNAs to a single LhrC molecule. Finally, we found that LhrC contributes to infection in macrophage-like cells. These findings demonstrate a central role for LhrC in controlling the level of OppA and other virulence-associated cell envelope proteins in response to cell envelope stress.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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