{"data":{"id":"10.6084/m9.figshare.1483475","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1483475","prefix":"10.6084","suffix":"m9.figshare.1483475","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Osterman, Ilya A","nameType":"Personal","givenName":"Ilya A","familyName":"Osterman","affiliation":[],"nameIdentifiers":[]},{"name":"Evfratov, Sergey A","nameType":"Personal","givenName":"Sergey A","familyName":"Evfratov","affiliation":[],"nameIdentifiers":[]},{"name":"Dzama, Margarita M","nameType":"Personal","givenName":"Margarita M","familyName":"Dzama","affiliation":[],"nameIdentifiers":[]},{"name":"Pletnev, Philipp I","nameType":"Personal","givenName":"Philipp I","familyName":"Pletnev","affiliation":[],"nameIdentifiers":[]},{"name":"Kovalchuk, Sergey I","nameType":"Personal","givenName":"Sergey I","familyName":"Kovalchuk","affiliation":[],"nameIdentifiers":[]},{"name":"Butenko, Ivan O","nameType":"Personal","givenName":"Ivan O","familyName":"Butenko","affiliation":[],"nameIdentifiers":[]},{"name":"Pobeguts, Olga V","nameType":"Personal","givenName":"Olga V","familyName":"Pobeguts","affiliation":[],"nameIdentifiers":[]},{"name":"Golovina, Anna Ya","nameType":"Personal","givenName":"Anna Ya","familyName":"Golovina","affiliation":[],"nameIdentifiers":[]},{"name":"Govorun, Vadim M","nameType":"Personal","givenName":"Vadim M","familyName":"Govorun","affiliation":[],"nameIdentifiers":[]},{"name":"Bogdanov, Alexey A","nameType":"Personal","givenName":"Alexey A","familyName":"Bogdanov","affiliation":[],"nameIdentifiers":[]},{"name":"Sergiev, Petr V","nameType":"Personal","givenName":"Petr V","familyName":"Sergiev","affiliation":[],"nameIdentifiers":[]},{"name":"Dontsova, Olga A","nameType":"Personal","givenName":"Olga A","familyName":"Dontsova","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A bacterial homolog YciH of eukaryotic translation initiation factor eIF1 regulates stress-related gene expression and is unlikely to be involved in translation initiation fidelity"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"Biological Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-07-15","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1069464","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["809472 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"YciH is a bacterial protein, homologous to eukaryotic translation initiation factor eIF1. Preceding evidence obtained with the aid of \u003ci\u003ein vitro\u003c/i\u003e translation initiation system suggested that it may play a role of a translation initiation factor, ensuring selection against suboptimal initiation complexes. Here we studied the effect of \u003ci\u003eEscherichia coli yciH\u003c/i\u003e gene inactivation on translation of model mRNAs. Neither the translation efficiency of leaderless mRNAs, nor mRNAs with non AUG start codons, was found to be affected by YciH \u003ci\u003ein vivo\u003c/i\u003e. Comparative proteome analysis revealed that \u003ci\u003eyciH\u003c/i\u003e gene knockout leads to a more than fold2- increase in expression of 66 genes and a more than fold2- decrease in the expression of 20 genes. Analysis of these gene sets allowed us to suggest a role of YciH as an inhibitor of translation in a stress response rather than the role of a translation initiation factor.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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