{"data":{"id":"10.6084/m9.figshare.1482037.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1482037.v1","prefix":"10.6084","suffix":"m9.figshare.1482037.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yang, Fan","givenName":"Fan","familyName":"Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Abdelnabby, Hazem","givenName":"Hazem","familyName":"Abdelnabby","affiliation":[],"nameIdentifiers":[]},{"name":"Xiao, Yannong","givenName":"Yannong","familyName":"Xiao","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The Zn(II)2Cys6 putative transcription factor is involved in the regulation of leucinostatin production and pathogenicity of the nematophagous fungus \u003ci\u003ePaecilomyces lilacinus\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Computational Biology"},{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Meta Science"},{"subject":"Ecology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"}],"contributors":[],"dates":[{"date":"2015-07-03","dateType":"Created"},{"date":"2023-06-28","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1482037","relatedIdentifierType":"DOI"},{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/07060661.2015.1065437","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["8050303 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Zn(II)2Cys6 transcription factor genes encode transcription regulators that manage the infection potential and production of secondary metabolites such as toxins in fungi. In this study, a gene named \u003ci\u003erolP\u003c/i\u003e that encodes a putative Zn(II)2Cys6 transcription factor regulating leucinostatin production in the filamentous fungus \u003ci\u003ePaecilomyces lilacinus\u003c/i\u003e was characterized by a gene knockout approach. The deduced proprotein consists of 705 amino acids and is highly homologous to the Zn(II)2Cys6 transcription factor of the entomopathogenic fungus \u003ci\u003eMetarhizium brunneum\u003c/i\u003e. Predictive analysis of the secondary structure of the proportion showed that it has two domains. \u003ci\u003ePaecilomyces lilacinus\u003c/i\u003e can produce nematotoxins leucinostatins A and B. Deletion of \u003ci\u003erolP\u003c/i\u003e from the \u003ci\u003eP. lilacinus\u003c/i\u003e wild-type strain Pl36-1 leads to the absence of leucinostatin A, while a large increase in the leucinostatin A level was achieved in the \u003ci\u003erolP\u003c/i\u003e overexpression strain Ov-Pl36-1. During the process of nematode infection, \u003ci\u003erolP\u003c/i\u003e showed high expression levels at 48–72 h and peaked at 48 h. Bioassay tests confirmed the requirement of \u003ci\u003erolP\u003c/i\u003e for leucinostatin production. However, the root-knot second-stage juvenile-toxicity was minimized to 35.3% in Δ\u003ci\u003erolP\u003c/i\u003e; toxicity was 97.2% with Ov-Pl36-1 compared with 68.4% with the wild type strain Pl36-1. Interestingly, neither knockout nor overexpression of the \u003ci\u003erolP\u003c/i\u003e gene affected the growth or sporulation of \u003ci\u003eP. lilacinus\u003c/i\u003e. Additionally, fungal nutrition and acidic media might stimulate the \u003ci\u003erolP\u003c/i\u003e activity of the wild type strain against nematodes. These findings suggested that the \u003ci\u003erolP\u003c/i\u003e gene is required for the induction and production of leucinostatins and is considered a leucinostatin regulatory gene in \u003ci\u003eP. lilacinus\u003c/i\u003e.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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