{"data":{"id":"10.6084/m9.figshare.1471677","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1471677","prefix":"10.6084","suffix":"m9.figshare.1471677","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Holst, Frederik","nameType":"Personal","givenName":"Frederik","familyName":"Holst","affiliation":[],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-5928-2175","nameIdentifierScheme":"ORCID"}]},{"name":"Ruiz, Christian","nameType":"Personal","givenName":"Christian","familyName":"Ruiz","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Video Clip S1+S2"}],"publisher":"figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Diseases"},{"subject":"Pathology"},{"subject":"Computer Software"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Computer and information sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Medicine"},{"subject":"60102 Bioinformatics","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"Pathogenesis"},{"subject":"Data Format"},{"subject":"Health Care"},{"subject":"Biological Techniques"},{"subject":"Computational Biology"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Applied Computer Science"},{"subject":"Biomarkers"}],"contributors":[],"dates":[{"date":"2015-07-02","dateType":"Created"},{"date":"2016-03-27","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["249229774 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003cstrong\u003eEstrogen receptor alpha (\u003cem\u003eESR1\u003c/em\u003e) gene amplification is frequent in breast cancer. Subsequent Supplementary Material.\u003c/strong\u003e Frederik Holst \u0026amp; Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (\u003cem\u003eESR1\u003c/em\u003e) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). Subsequent Supplementary Figures S1-S7 Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented \u003cem\u003eESR1\u003c/em\u003e copy number profiles of 22 breast cancers. A photo documentation of ESR1 FISH analysis signal appearance (figures 5A-C + 7A-C) of tumors with \u003cem\u003eESR1\u003c/em\u003e amplification by SNP GeneChip analysis is added. DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 Subsequent Supplementary Video Clips S1+S2 The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 Subsequent Supplementary Optical Dataset S1 The optical dataset consists of 94 photographs documenting appearance of \u003cem\u003eESR1\u003c/em\u003e FISH analysis signals of 20 out of 22 tumors regarding SNP GeneChip analysis illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 \u003cstrong\u003eMethods References\u003c/strong\u003e Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. \u0026amp; van Diest, P.J. \u003cem\u003eESR1\u003c/em\u003e Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of \u003cem\u003eESR1\u003c/em\u003e Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 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