{"data":{"id":"10.6084/m9.figshare.1470130","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1470130","prefix":"10.6084","suffix":"m9.figshare.1470130","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Holst, Frederik","nameType":"Personal","givenName":"Frederik","familyName":"Holst","affiliation":[],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-5928-2175","nameIdentifierScheme":"ORCID"}]},{"name":"Ruiz, Christian","nameType":"Personal","givenName":"Christian","familyName":"Ruiz","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent supplementary optical dataset S1"}],"publisher":"figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Diseases"},{"subject":"Pathology"},{"subject":"Computer Software"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Computer and information sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Medicine"},{"subject":"60102 Bioinformatics","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"Pathogenesis"},{"subject":"Data Format"},{"subject":"Health Care"},{"subject":"Biological Techniques"},{"subject":"Computational Biology"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Applied Computer Science"},{"subject":"Biomarkers"}],"contributors":[],"dates":[{"date":"2015-07-01","dateType":"Created"},{"date":"2016-03-27","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["17018044 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003cstrong\u003eEstrogen receptor alpha (\u003cem\u003eESR1\u003c/em\u003e) gene amplification is frequent in breast cancer. Subsequent supplementary material.\u003c/strong\u003e Frederik Holst \u0026amp; Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (\u003cem\u003eESR1\u003c/em\u003e) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). \u003cstrong\u003eSubsequent Supplementary Figures S1-S7\u003c/strong\u003e Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented \u003cem\u003eESR1\u003c/em\u003e copy number profiles of 22 breast cancers. Taken from Subsequent Supplementary Optical Dataset S1 a photographical documentation of \u003cem\u003eESR1\u003c/em\u003e FISH analysis signal appearance of tumors with \u003cem\u003eESR1\u003c/em\u003e amplification by SNP GeneChip analysis is added (figures 5A-C + 7A-C). DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 \u003cstrong\u003eSubsequent Supplementary Video Clips S1+S2\u003c/strong\u003e The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. Video clips are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 x 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 \u003cstrong\u003eSubsequent Supplementary Optical Dataset S1\u003c/strong\u003e The optical dataset consists of 94 photographs that show the appearance of \u003ci\u003eESR1\u003c/i\u003e FISH signals of 20 out of 22 tumors in regard to the GeneChip analysis that is illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. Photographs are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 \u003cstrong\u003eMethods References\u003c/strong\u003e Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. \u0026amp; van Diest, P.J. \u003cem\u003eESR1\u003c/em\u003e Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of \u003cem\u003eESR1\u003c/em\u003e Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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