{"data":{"id":"10.6084/m9.figshare.1470035","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1470035","prefix":"10.6084","suffix":"m9.figshare.1470035","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Holst, Frederik","nameType":"Personal","givenName":"Frederik","familyName":"Holst","affiliation":[],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-5928-2175","nameIdentifierScheme":"ORCID"}]},{"name":"Ruiz, Christian","nameType":"Personal","givenName":"Christian","familyName":"Ruiz","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Figures S1-S7"}],"publisher":"figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Diseases"},{"subject":"Pathology"},{"subject":"Computer Software"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Computer and information sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Medicine"},{"subject":"60102 Bioinformatics","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"Pathogenesis"},{"subject":"Data Format"},{"subject":"Health Care"},{"subject":"Biological Techniques"},{"subject":"Computational Biology"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Applied Computer Science"},{"subject":"Biomarkers"}],"contributors":[],"dates":[{"date":"2015-07-01","dateType":"Created"},{"date":"2016-03-30","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["1937503 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003cstrong\u003eEstrogen receptor alpha (\u003cem\u003eESR1\u003c/em\u003e) gene amplification is frequent in breast cancer. Subsequent supplementary material.\u003c/strong\u003e Frederik Holst \u0026amp; Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (\u003cem\u003eESR1\u003c/em\u003e) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). \u003cstrong\u003eSubsequent Supplementary Figures S1-S7\u003c/strong\u003e Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented \u003cem\u003eESR1\u003c/em\u003e copy number profiles of 22 breast cancers. Taken from Subsequent Supplementary Optical Dataset S1 a photographical documentation of \u003cem\u003eESR1\u003c/em\u003e FISH analysis signal appearance of tumors with \u003cem\u003eESR1\u003c/em\u003e amplification by SNP GeneChip analysis is added (figures 5A-C + 7A-C). DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 \u003cstrong\u003eSubsequent Supplementary Video Clips S1+S2\u003c/strong\u003e The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. Video clips are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 x 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 \u003cstrong\u003eSubsequent Supplementary Optical Dataset S1\u003c/strong\u003e The optical dataset consists of 94 photographs that show the appearance of \u003ci\u003eESR1\u003c/i\u003e FISH signals of 20 out of 22 tumors in regard to the GeneChip analysis that is illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. Photographs are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 \u003cstrong\u003eMethods References\u003c/strong\u003e Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. \u0026amp; van Diest, P.J. \u003cem\u003eESR1\u003c/em\u003e Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of \u003cem\u003eESR1\u003c/em\u003e Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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