{"data":{"id":"10.6084/m9.figshare.1456233.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1456233.v1","prefix":"10.6084","suffix":"m9.figshare.1456233.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wa Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Barger, Carter J","nameType":"Personal","givenName":"Carter J","familyName":"Barger","affiliation":[],"nameIdentifiers":[]},{"name":"Link, Petra A","nameType":"Personal","givenName":"Petra A","familyName":"Link","affiliation":[],"nameIdentifiers":[]},{"name":"Mhawech-Fauceglia, Paulette","nameType":"Personal","givenName":"Paulette","familyName":"Mhawech-Fauceglia","affiliation":[],"nameIdentifiers":[]},{"name":"Miller, Austin","nameType":"Personal","givenName":"Austin","familyName":"Miller","affiliation":[],"nameIdentifiers":[]},{"name":"Akers, Stacey N","nameType":"Personal","givenName":"Stacey N","familyName":"Akers","affiliation":[],"nameIdentifiers":[]},{"name":"Kunle Odunsi","affiliation":[],"nameIdentifiers":[]},{"name":"Karpf, Adam R","nameType":"Personal","givenName":"Adam R","familyName":"Karpf","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"DNA hypomethylation-mediated activation of \u003ci\u003eCancer/Testis Antigen 45\u003c/i\u003e (\u003ci\u003eCT45\u003c/i\u003e) genes is associated with disease progression and reduced survival in epithelial ovarian cancer"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cancer"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-08-03","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15592294.2015.1062206","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1456233","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["566956 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"ABSTRACTEpithelial ovarian cancer (EOC) is a highly lethal malignancy due to a lack of early detection approaches coupled with poor outcomes for patients with clinically advanced disease. Cancer-testis (CT) or cancer-germline genes encode antigens known to generate spontaneous anti-tumor immunity in cancer patients. CT45 genes are a recently discovered six-member family of X-linked CT genes with oncogenic function. Here, we determined CT45 expression in EOC and fully defined its epigenetic regulation by DNA methylation. \u003ci\u003eCT45\u003c/i\u003e was silent and hypermethylated in normal control tissues, but a large subset of EOC samples showed increased \u003ci\u003eCT45\u003c/i\u003e expression in conjunction with promoter DNA hypomethylation. In contrast, copy number status did not correlate with \u003ci\u003eCT45\u003c/i\u003e expression in the TCGA database for EOC. \u003ci\u003eCT45\u003c/i\u003e promoter methylation inversely correlated with both CT45 mRNA and protein expression, the latter determined using IHC staining of an EOC TMA. \u003ci\u003eCT45\u003c/i\u003e expression was increased and \u003ci\u003eCT45\u003c/i\u003e promoter methylation was decreased in late-stage and high-grade EOC, and both measures were associated with poor survival. \u003ci\u003eCT45\u003c/i\u003e hypomethylation was directly associated with \u003ci\u003eLINE-1\u003c/i\u003e hypomethylation, and \u003ci\u003eCT45\u003c/i\u003e was frequently co-expressed with other CT antigen genes in EOC. Decitabine treatment induced CT45 mRNA and protein expression in EOC cells, and promoter transgene analyses indicated that DNA methylation directly represses \u003ci\u003eCT45\u003c/i\u003e promoter activity. These data verify CT45 expression and promoter hypomethylation as possible prognostic biomarkers, and suggest CT45 as an immunological or therapeutic target in EOC. Treatment with decitabine or other epigenetic modulators could provide a means for more effective immunological targeting of CT45.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/DNA_hypomethylation_mediated_activation_of_i_Cancer_Testis_Antigen_45_i_i_CT45_i_genes_is_associated_with_disease_progression_and_reduced_survival_in_epithelial_ovarian_cancer/1456233/1","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T23:32:50.000Z","registered":"2016-01-19T23:32:51.000Z","published":"2015","updated":"2020-09-04T16:54:15.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1456233.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}