{"data":{"id":"10.6084/m9.figshare.1454499","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1454499","prefix":"10.6084","suffix":"m9.figshare.1454499","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jiantang Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Aifen Tao","affiliation":[],"nameIdentifiers":[]},{"name":"Liwu Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Pingping Fang","affiliation":[],"nameIdentifiers":[]},{"name":"Lihui Lin","affiliation":[],"nameIdentifiers":[]},{"name":"Jianmin Qi","affiliation":[],"nameIdentifiers":[]},{"name":"Gaoyang Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Yujia Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Feng Tao","nameType":"Personal","givenName":"Feng Tao","familyName":"Li","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"An Efficient Regeneration System and Optimization of the Transformation from the Cotyledonary Node of Jute (\u003ci\u003eCorchorus capsularis\u003c/i\u003e L.)"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Neuroscience"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-06-19","dateType":"Created"},{"date":"2015-06-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15440478.2014.912972","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["25600 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The purpose of this study was to use cotyledonary nodes as explants to establish an efficient regeneration protocol for jute (\u003ci\u003eCorchorus capsularis\u003c/i\u003e L.) on Murashige and Skoog (MS) basal medium. Research into the different growth regulators using the orthogonal design L16 (4\u003csup\u003e5\u003c/sup\u003e) revealed that the best shoot induction medium is MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6BA and 0.25 mg/L NAA. The average number of shoots per explant and the explant induction rate were 9.8 and 100%. After 3 weeks, 2–3 cm shoots were rooting on 1/2 MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6-BA + 0.2 mg/L NAA. Moreover, we optimized \u003ci\u003eAgrobacterium\u003c/i\u003e-mediated transformation using the \u003ci\u003eGUS\u003c/i\u003e gene transient expression system. The best condition for obtaining higher transformation rate consisted of the use of fresh explants to which 100 μM acetosyringone was added for a co-culture time of 10 min, the OD value of \u003ci\u003eAgrobacterium\u003c/i\u003e liquid is 0.5 at 600 nm. These data provide an important basis for the application of other trait gene in the improvement of jute fiber quality.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com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