{"data":{"id":"10.6084/m9.figshare.1423299","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1423299","prefix":"10.6084","suffix":"m9.figshare.1423299","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Giraud, Caroline","nameType":"Personal","givenName":"Caroline","familyName":"Giraud","affiliation":[],"nameIdentifiers":[]},{"name":"Hausmann, Stéphane","nameType":"Personal","givenName":"Stéphane","familyName":"Hausmann","affiliation":[],"nameIdentifiers":[]},{"name":"Lemeille, Sylvain","nameType":"Personal","givenName":"Sylvain","familyName":"Lemeille","affiliation":[],"nameIdentifiers":[]},{"name":"Prados, Julien","nameType":"Personal","givenName":"Julien","familyName":"Prados","affiliation":[],"nameIdentifiers":[]},{"name":"Redder, Peter","nameType":"Personal","givenName":"Peter","familyName":"Redder","affiliation":[],"nameIdentifiers":[]},{"name":"Linder, Patrick","nameType":"Personal","givenName":"Patrick","familyName":"Linder","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The C-terminal region of the RNA helicase CshA is required for the interaction with the degradosome and turnover of bulk RNA in the opportunistic pathogen \u003ci\u003eStaphylococcus aureus\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-05-22","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1035505","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2794583 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eStaphylococcus aureus\u003c/i\u003e is a versatile opportunistic pathogen that adapts readily to a variety of different growth conditions. This adaptation requires a rapid regulation of gene expression including the control of mRNA abundance. The CshA DEAD-box RNA helicase was previously shown to be required for efficient turnover of the \u003ci\u003eagr\u003c/i\u003e quorum sensing mRNA. Here we show by transcriptome-wide RNA sequencing and microarray analyses that CshA is required for the degradation of bulk mRNA. Moreover a subset of mRNAs is significantly stabilised in absence of CshA. Deletion of the C-terminal extension affects RNA turnover similar to the full deletion of the \u003ci\u003ecshA\u003c/i\u003e gene. In accordance with RNA decay data, the C-terminal region of CshA is required for an RNA-independent interaction with components of the RNA degradation machinery. The C-terminal truncation of CshA reduces its ATPase activity and this reduction cannot be compensated at high RNA concentrations. Finally, the deletion of the C-terminal extension does affect growth at low temperatures, but to a significantly lesser degree than the full deletion, indicating that the core of the helicase can assume a partial function and opening the possibility that CshA is involved in different cellular processes.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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