{"data":{"id":"10.6084/m9.figshare.1422054.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1422054.v3","prefix":"10.6084","suffix":"m9.figshare.1422054.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Darawan Rinchai","affiliation":[],"nameIdentifiers":[]},{"name":"Donporn Riyapa","affiliation":[],"nameIdentifiers":[]},{"name":"Surachat Buddhisa","affiliation":[],"nameIdentifiers":[]},{"name":"Kusumawadee Utispan","affiliation":[],"nameIdentifiers":[]},{"name":"Titball, Richard W","nameType":"Personal","givenName":"Richard W","familyName":"Titball","affiliation":[],"nameIdentifiers":[]},{"name":"Stevens, Mark P","nameType":"Personal","givenName":"Mark P","familyName":"Stevens","affiliation":[],"nameIdentifiers":[]},{"name":"Stevens, Joanne M","nameType":"Personal","givenName":"Joanne M","familyName":"Stevens","affiliation":[],"nameIdentifiers":[]},{"name":"Michinaga Ogawa","affiliation":[],"nameIdentifiers":[]},{"name":"Isei Tanida","affiliation":[],"nameIdentifiers":[]},{"name":"Koike, Masato","nameType":"Personal","givenName":"Masato","familyName":"Koike","affiliation":[],"nameIdentifiers":[]},{"name":"Uchiyama, Yasuo","nameType":"Personal","givenName":"Yasuo","familyName":"Uchiyama","affiliation":[],"nameIdentifiers":[]},{"name":"Ato, Manabu","nameType":"Personal","givenName":"Manabu","familyName":"Ato","affiliation":[],"nameIdentifiers":[]},{"name":"Ganjana Lertmemongkolchai","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Macroautophagy is essential for killing of intracellular \u003ci\u003eBurkholderia pseudomallei\u003c/i\u003e in human neutrophils"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Physiology"},{"subject":"Cell Biology"},{"subject":"Microbiology"}],"contributors":[],"dates":[{"date":"2015-07-17","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15548627.2015.1040969","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1422054","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["5700625 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Neutrophils play a key role in the control of \u003ci\u003eBurkholderia pseudomallei\u003c/i\u003e, the pathogen that causes melioidosis. Here, we show that survival of intracellular \u003ci\u003eB. pseudomallei\u003c/i\u003e was significantly increased in the presence of 3-methyladenine or lysosomal cathepsin inhibitors. The LC3-flux was increased in \u003ci\u003eB. pseudomallei\u003c/i\u003e-infected neutrophils. Concordant with this result, confocal microscopy analyses using anti-LC3 antibodies revealed that \u003ci\u003eB. pseudomallei\u003c/i\u003e-containing phagosomes partially overlapped with LC3-positive signal at 3 and 6 h postinfection. Electron microscopic analyses of \u003ci\u003eB. pseudomallei\u003c/i\u003e-infected neutrophils at 3 h revealed \u003ci\u003eB. pseudomallei\u003c/i\u003e-containing phagosomes that occasionally fused with phagophores or autophagosomes. Following infection with a \u003ci\u003eB. pseudomallei\u003c/i\u003e mutant lacking the \u003ci\u003eBurkholderia\u003c/i\u003e secretion apparatus Bsa Type III secretion system, neither this characteristic structure nor bacterial escape into the cytosol were observed. These findings indicate that human neutrophils are able to recruit autophagic machinery adjacent to \u003ci\u003eB. pseudomallei\u003c/i\u003e-containing phagosomes in a Type III secretion system-dependent manner.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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