{"data":{"id":"10.6084/m9.figshare.1405689.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1405689.v2","prefix":"10.6084","suffix":"m9.figshare.1405689.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Miwa, Shinji","nameType":"Personal","givenName":"Shinji","familyName":"Miwa","affiliation":[],"nameIdentifiers":[]},{"name":"Shuya Yano","affiliation":[],"nameIdentifiers":[]},{"name":"Kimura, Hiroaki","nameType":"Personal","givenName":"Hiroaki","familyName":"Kimura","affiliation":[],"nameIdentifiers":[]},{"name":"Yamamoto, Mako","nameType":"Personal","givenName":"Mako","familyName":"Yamamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Toneri, Makoto","nameType":"Personal","givenName":"Makoto","familyName":"Toneri","affiliation":[],"nameIdentifiers":[]},{"name":"Murakami, Takashi","nameType":"Personal","givenName":"Takashi","familyName":"Murakami","affiliation":[],"nameIdentifiers":[]},{"name":"Hayashi, Katsuhiro","nameType":"Personal","givenName":"Katsuhiro","familyName":"Hayashi","affiliation":[],"nameIdentifiers":[]},{"name":"Yamamoto, Norio","nameType":"Personal","givenName":"Norio","familyName":"Yamamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Toshiyoshi Fujiwara","affiliation":[],"nameIdentifiers":[]},{"name":"Tsuchiya, Hiroyuki","nameType":"Personal","givenName":"Hiroyuki","familyName":"Tsuchiya","affiliation":[],"nameIdentifiers":[]},{"name":"Hoffman, Robert M","nameType":"Personal","givenName":"Robert M","familyName":"Hoffman","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Physics"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-07-07","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1033598","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1405689","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["13939303 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The present study analyzed the heterogeneous cell-cycle dependence and fate of single cancer cells in a population treated with UVB using a fluorescence ubiquitination-based cell-cycle (FUCCI) imaging system. HeLa cells expressing FUCCI were irradiated by 100 or 200 J/m\u003csup\u003e2\u003c/sup\u003e UVB. Modulation of the cell-cycle and apoptosis were observed by time-lapse confocal microscopy imaging every 30 min for 72 h. Correlation between cell survival and factors including cell-cycle phase at the time of the irradiation of UVB, mitosis and the G\u003csub\u003e1\u003c/sub\u003e/S transition were analyzed using the Kaplan–Meier method along with the log rank test. Time-lapse FUCCI imaging of HeLa cells demonstrated that UVB irradiation induced cell-cycle arrest in S/G\u003csub\u003e2\u003c/sub\u003e/M phase in the majority of the cells. The cells irradiated by 100 or 200 J/m\u003csup\u003e2\u003c/sup\u003e UVB during G\u003csub\u003e0\u003c/sub\u003e/G\u003csub\u003e1\u003c/sub\u003e phase had a higher survival rate than the cells irradiated during S/G\u003csub\u003e2\u003c/sub\u003e/M phase. A minority of cells could escape S/G\u003csub\u003e2\u003c/sub\u003e/M arrest and undergo mitosis which significantly correlated with decreased survival of the cells. In contrast, G\u003csub\u003e1\u003c/sub\u003e/S transition significantly correlated with increased survival of the cells after UVB irradiation. UVB at 200 J/m\u003csup\u003e2\u003c/sup\u003e resulted in a greater number of apoptotic cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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