{"data":{"id":"10.6084/m9.figshare.1405689","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1405689","prefix":"10.6084","suffix":"m9.figshare.1405689","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Miwa, Shinji","nameType":"Personal","givenName":"Shinji","familyName":"Miwa","affiliation":[],"nameIdentifiers":[]},{"name":"Shuya Yano","affiliation":[],"nameIdentifiers":[]},{"name":"Kimura, Hiroaki","nameType":"Personal","givenName":"Hiroaki","familyName":"Kimura","affiliation":[],"nameIdentifiers":[]},{"name":"Yamamoto, Mako","nameType":"Personal","givenName":"Mako","familyName":"Yamamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Toneri, Makoto","nameType":"Personal","givenName":"Makoto","familyName":"Toneri","affiliation":[],"nameIdentifiers":[]},{"name":"Murakami, Takashi","nameType":"Personal","givenName":"Takashi","familyName":"Murakami","affiliation":[],"nameIdentifiers":[]},{"name":"Hayashi, Katsuhiro","nameType":"Personal","givenName":"Katsuhiro","familyName":"Hayashi","affiliation":[],"nameIdentifiers":[]},{"name":"Yamamoto, Norio","nameType":"Personal","givenName":"Norio","familyName":"Yamamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Toshiyoshi Fujiwara","affiliation":[],"nameIdentifiers":[]},{"name":"Tsuchiya, Hiroyuki","nameType":"Personal","givenName":"Hiroyuki","familyName":"Tsuchiya","affiliation":[],"nameIdentifiers":[]},{"name":"Hoffman, Robert M","nameType":"Personal","givenName":"Robert M","familyName":"Hoffman","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Biotechnology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2015-05-06","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1033598","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["13939303 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The present study analyzed the heterogeneous cell-cycle dependence and fate of single cancer cells in a population treated with UVB using a fluorescence ubiquitination-based cell-cycle (FUCCI) imaging system. HeLa cells expressing FUCCI were irradiated by 100 or 200 J/m\u003csup\u003e2\u003c/sup\u003e UVB. Modulation of the cell-cycle and apoptosis were observed by time-lapse confocal microscopy imaging every 30 min for 72 h. Correlation between cell survival and factors including cell-cycle phase at the time of the irradiation of UVB, mitosis and the G\u003csub\u003e1\u003c/sub\u003e/S transition were analyzed using the Kaplan–Meier method along with the log rank test. Time-lapse FUCCI imaging of HeLa cells demonstrated that UVB irradiation induced cell-cycle arrest in S/G\u003csub\u003e2\u003c/sub\u003e/M phase in the majority of the cells. The cells irradiated by 100 or 200 J/m\u003csup\u003e2\u003c/sup\u003e UVB during G\u003csub\u003e0\u003c/sub\u003e/G\u003csub\u003e1\u003c/sub\u003e phase had a higher survival rate than the cells irradiated during S/G\u003csub\u003e2\u003c/sub\u003e/M phase. A minority of cells could escape S/G\u003csub\u003e2\u003c/sub\u003e/M arrest and undergo mitosis which significantly correlated with decreased survival of the cells. In contrast, G\u003csub\u003e1\u003c/sub\u003e/S transition significantly correlated with increased survival of the cells after UVB irradiation. UVB at 200 J/m\u003csup\u003e2\u003c/sup\u003e resulted in a greater number of apoptotic cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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