{"data":{"id":"10.6084/m9.figshare.1399157.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1399157.v3","prefix":"10.6084","suffix":"m9.figshare.1399157.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Chenshu Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Jen-Zen Chuang","affiliation":[],"nameIdentifiers":[]},{"name":"Ching-Hwa Sung","affiliation":[],"nameIdentifiers":[]},{"name":"Yinghui Mao","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A dynein independent role of Tctex-1 at the kinetochore"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"}],"contributors":[],"dates":[{"date":"2015-10-09","dateType":"Created"},{"date":"2018-08-31","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2014.1000217","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1399157","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["11933595 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Dynein light chains are accessory subunits of the cytoplasmic dynein complex, a minus-end directed microtubule motor. Here, we demonstrate that the dynein light chain Tctex-1 associates with unattached kinetochores and is essential for accurate chromosome segregation. Tctex-1 knockdown in cells does not affect the localization and function of dynein at the kinetochore, but produces a prolonged mitotic arrest with a few misaligned chromosomes, which are subsequently missegregated during anaphase. This function is independent of Tctex-1's association with dynein. The kinetochore localization of Tctex-1 is independent of the ZW10-dynein pathway, but requires the Ndc80 complex. Thus, our findings reveal a dynein independent role of Tctex-1 at the kinetochore to enhance the stability of kinetochore-microtubule attachment.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/A_dynein_independent_role_of_Tctex_1_at_the_kinetochore_/1399157/3","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T20:19:03.000Z","registered":"2016-01-19T20:19:04.000Z","published":"2015","updated":"2020-09-04T17:48:45.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1399157.v3","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}