{"data":{"id":"10.6084/m9.figshare.1384857","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1384857","prefix":"10.6084","suffix":"m9.figshare.1384857","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Castillo, Daniela S","nameType":"Personal","givenName":"Daniela S","familyName":"Castillo","affiliation":[],"nameIdentifiers":[]},{"name":"Campalans, Anna","nameType":"Personal","givenName":"Anna","familyName":"Campalans","affiliation":[],"nameIdentifiers":[]},{"name":"Belluscio, Laura M","nameType":"Personal","givenName":"Laura M","familyName":"Belluscio","affiliation":[],"nameIdentifiers":[]},{"name":"Carcagno, Abel L","nameType":"Personal","givenName":"Abel L","familyName":"Carcagno","affiliation":[],"nameIdentifiers":[]},{"name":"J Pablo Radicella","affiliation":[],"nameIdentifiers":[]},{"name":"Cánepa, Eduardo T","nameType":"Personal","givenName":"Eduardo T","familyName":"Cánepa","affiliation":[],"nameIdentifiers":[]},{"name":"Pregi, Nicolás","nameType":"Personal","givenName":"Nicolás","familyName":"Pregi","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"E2F1 and E2F2 induction in response to DNA damage preserves genomic stability in neuronal cells"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2015-04-18","dateType":"Created"},{"date":"2019-04-02","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15384101.2014.985031","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["7016778 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"E2F transcription factors regulate a wide range of biological processes, including the cellular response to DNA damage. In the present study, we examined whether E2F family members are transcriptionally induced following treatment with several genotoxic agents, and have a role on the cell DNA damage response. We show a novel mechanism, conserved among diverse species, in which \u003ci\u003eE2F1\u003c/i\u003e and \u003ci\u003eE2F2\u003c/i\u003e, the latter specifically in neuronal cells, are transcriptionally induced after DNA damage. This upregulation leads to increased E2F1 and E2F2 protein levels as a consequence of \u003ci\u003ede novo\u003c/i\u003e protein synthesis. Ectopic expression of these E2Fs in neuronal cells reduces the level of DNA damage following genotoxic treatment, while ablation of E2F1 and E2F2 leads to the accumulation of DNA lesions and increased apoptotic response. Cell viability and DNA repair capability in response to DNA damage induction are also reduced by the E2F1 and E2F2 deficiencies. Finally, E2F1 and E2F2 accumulate at sites of oxidative and UV-induced DNA damage, and interact with γH2AX DNA repair factor. As previously reported for E2F1, E2F2 promotes Rad51 foci formation, interacts with GCN5 acetyltransferase and induces histone acetylation following genotoxic insult. The results presented here unveil a new mechanism involving E2F1 and E2F2 in the maintenance of genomic stability in response to DNA damage in neuronal cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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