{"data":{"id":"10.6084/m9.figshare.1379989","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1379989","prefix":"10.6084","suffix":"m9.figshare.1379989","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Sakamoto, Yamato","nameType":"Personal","givenName":"Yamato","familyName":"Sakamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Nakamoto, Yumi","nameType":"Personal","givenName":"Yumi","familyName":"Nakamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Tetsuyuki Wada","affiliation":[],"nameIdentifiers":[]},{"name":"Ichida, Seiji","nameType":"Personal","givenName":"Seiji","familyName":"Ichida","affiliation":[],"nameIdentifiers":[]},{"name":"Minami, Takeshi","nameType":"Personal","givenName":"Takeshi","familyName":"Minami","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Ethyl mercury induces Ca\u003csup\u003e2+\u003c/sup\u003e uptake through the P2×7 receptor in a mouse cerebellar microglia cell line"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Pharmacology"},{"subject":"Neuroscience"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-04-15","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/02772248.2015.1028407","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["334168 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Many observations are reported that organic mercury compounds are involved in increasing intracellular Ca\u003csup\u003e2+\u003c/sup\u003e levels. However, the issue of which substances on the cell membrane participate in the Ca\u003csup\u003e2+\u003c/sup\u003e uptake that is induced by ethyl mercury is unclear. The findings of this study suggest that the P2X receptor participates in this process. The uptake of Ca\u003csup\u003e2+\u003c/sup\u003e by C8-B4 cells was induced in the presence of ethyl mercury. Ca channels in the cell membrane were not affected in this process. In contrast, pretreatment with suramin, an antagonist of the P2X receptor, inhibited the Ca\u003csup\u003e2+\u003c/sup\u003e uptake induced by ethyl mercury, and also brilliant blue G, a nonselective antagonist of P2×4, P2×5, and P2×7 receptors. In addition, A438079 and A740003, selective antagonists of P2×7 receptor, reduced Ca\u003csup\u003e2+\u003c/sup\u003e uptake, while 5-BDBD, a selective antagonist of P2×4 receptor, did not. Furthermore, the mRNAs of both the P2×4 and P2×7 receptors were expressed in the presence of ethyl mercury, but the P2×5 receptor mRNA was not. These findings suggest that ethyl mercury may induce Ca\u003csup\u003e2+\u003c/sup\u003e uptake through the P2×7 receptor of the cell membrane.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Ethyl_mercury_induces_Ca_sup_2_sup_uptake_through_the_P2_215_7_receptor_in_a_mouse_cerebellar_microglia_cell_line/1379989","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"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