{"data":{"id":"10.6084/m9.figshare.1378876","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1378876","prefix":"10.6084","suffix":"m9.figshare.1378876","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Honda, Shozo","nameType":"Personal","givenName":"Shozo","familyName":"Honda","affiliation":[],"nameIdentifiers":[]},{"name":"Shigematsu, Megumi","nameType":"Personal","givenName":"Megumi","familyName":"Shigematsu","affiliation":[],"nameIdentifiers":[]},{"name":"Morichika, Keisuke","nameType":"Personal","givenName":"Keisuke","familyName":"Morichika","affiliation":[],"nameIdentifiers":[]},{"name":"Telonis, Aristeidis G","nameType":"Personal","givenName":"Aristeidis G","familyName":"Telonis","affiliation":[],"nameIdentifiers":[]},{"name":"Kirino, Yohei","nameType":"Personal","givenName":"Yohei","familyName":"Kirino","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Engineering"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-04-14","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1031951","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1032791 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Transfer RNAs (tRNAs) play a central role in translation and also recently appear to have a variety of other functions in biological processes beyond translation. Here we report the development of Four-Leaf clover qRT-PCR (FL-PCR), a convenient PCR-based method, which can specifically quantify individual mature tRNA species. In FL-PCR, T4 RNA ligase 2 specifically ligates a stem-loop adapter to mature tRNAs but not to precursor tRNAs or tRNA fragments. Subsequent TaqMan qRT-PCR amplifies only unmodified regions of the tRNA-adapter ligation products; therefore, FL-PCR quantification is not influenced by tRNA post-transcriptional modifications. FL-PCR has broad applicability for the quantification of various tRNAs in different cell types, and thus provides a much-needed simple method for analyzing tRNA abundance and heterogeneity.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Four_leaf_clover_qRT_PCR_A_convenient_method_for_selective_quantification_of_mature_tRNA/1378876","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-06-02T13:04:50.000Z","registered":"2015-06-02T13:04:51.000Z","published":"2015","updated":"2020-09-04T18:06:23.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9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