{"data":{"id":"10.6084/m9.figshare.1378856.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1378856.v3","prefix":"10.6084","suffix":"m9.figshare.1378856.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Inamine, Saki","nameType":"Personal","givenName":"Saki","familyName":"Inamine","affiliation":[],"nameIdentifiers":[]},{"name":"Onaga, Shoko","nameType":"Personal","givenName":"Shoko","familyName":"Onaga","affiliation":[],"nameIdentifiers":[]},{"name":"Ohnuma, Takayuki","nameType":"Personal","givenName":"Takayuki","familyName":"Ohnuma","affiliation":[],"nameIdentifiers":[]},{"name":"Tamo Fukamizo","affiliation":[],"nameIdentifiers":[]},{"name":"Toki Taira","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Purification, cDNA cloning, and characterization of LysM-containing plant chitinase from horsetail (\u003ci\u003eEquisetum arvense\u003c/i\u003e)"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"Chemistry"},{"subject":"Evolutionary Biology"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-07-28","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2015.1025693","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1378856","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["134550 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Chitinase-A (EaChiA), molecular mass 36 kDa, was purified from the vegetative stems of a horsetail (\u003ci\u003eEquisetum arvense\u003c/i\u003e) using a series of column chromatography. The N-terminal amino acid sequence of EaChiA was similar to the lysin motif (LysM). A cDNA encoding EaChiA was cloned by rapid amplification of cDNA ends and polymerase chain reaction. It consisted of 1320 nucleotides and encoded an open reading frame of 361 amino acid residues. The deduced amino acid sequence indicated that EaChiA is composed of a N-terminal LysM domain and a C-terminal plant class IIIb chitinase catalytic domain, belonging to the glycoside hydrolase family 18, linked by proline-rich regions. EaChiA has strong chitin-binding activity, however, no antifungal activity. This is the first report of a chitinase from Equisetopsida, a class of fern plants, and the second report of a LysM-containing chitinase from a plant.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Purification_cDNA_cloning_and_characterization_of_LysM_containing_plant_chitinase_from_horsetail_i_Equisetum_arvense_i_/1378856/3","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