{"data":{"id":"10.6084/m9.figshare.1378749.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1378749.v3","prefix":"10.6084","suffix":"m9.figshare.1378749.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jung-Chun Lin","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"RBM4-MEF2C network constitutes a feed-forward circuit that facilitates the differentiation of brown adipocytes"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Computational Biology"},{"subject":"Hematology"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Meta Science"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Physiology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"}],"contributors":[],"dates":[{"date":"2015-10-15","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1017213","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1378749","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["183758 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Myocyte enhancer factor 2c (MEF2C) is the MADS-box type transcription factor involved in the differentiation of cardiac and skeletal muscle and synaptic formation. Alternatively spliced transcripts of the \u003ci\u003eMEF2C\u003c/i\u003e gene were proven to encode isoforms which exert distinct functions in transcriptional regulation. During the differentiation of brown adipocytes, upregulated RBM4 enhanced skipping of the \u003ci\u003eMEF2C\u003c/i\u003eγ region which functions as a transcriptional repressor. The presence of an overexpressed MEF2Cγ- isoform in turn induced transcriptional activity of the \u003ci\u003eRBM4\u003c/i\u003e promoter, constituting a positive feedback circuit in differentiating brown adipocytes. The RBM4-MEF2Cγ- network induced the expression of \"myogenic\" miR-1 to a greater extent than did \u003ci\u003ePRDM17, BMP7 C/EBP\u003c/i\u003eβ, or \u003ci\u003eUCP1\u003c/i\u003e transcripts in C3H10T1/2 cells. Overexpression of miR-1 independently exerted the same activity as RBM4 and the MEF2Cγ- isoform of upregulating brown adipocyte-specific factors in C3H10T1/2 cells, which suggests a potential effect of miR-1 on brown adipocytes. These results indicated that the RBM4-MEF2C-miR-1 network constitutes a novel mechanism which programs the gene expression profile toward the development of brown adipocytes.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/RBM4_MEF2C_network_constitutes_a_feed_forward_circuit_that_facilitates_the_differentiation_of_brown_adipocytes/1378749/3","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"vers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