{"data":{"id":"10.6084/m9.figshare.1378746","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1378746","prefix":"10.6084","suffix":"m9.figshare.1378746","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Bibova, Ilona","nameType":"Personal","givenName":"Ilona","familyName":"Bibova","affiliation":[],"nameIdentifiers":[]},{"name":"Hot, David","nameType":"Personal","givenName":"David","familyName":"Hot","affiliation":[],"nameIdentifiers":[]},{"name":"Keidel, Kristina","nameType":"Personal","givenName":"Kristina","familyName":"Keidel","affiliation":[],"nameIdentifiers":[]},{"name":"Amman, Fabian","nameType":"Personal","givenName":"Fabian","familyName":"Amman","affiliation":[],"nameIdentifiers":[]},{"name":"Slupek, Stephanie","nameType":"Personal","givenName":"Stephanie","familyName":"Slupek","affiliation":[],"nameIdentifiers":[]},{"name":"Cerny, Ondrej","nameType":"Personal","givenName":"Ondrej","familyName":"Cerny","affiliation":[],"nameIdentifiers":[]},{"name":"Gross, Roy","nameType":"Personal","givenName":"Roy","familyName":"Gross","affiliation":[],"nameIdentifiers":[]},{"name":"Vecerek, Branislav","nameType":"Personal","givenName":"Branislav","familyName":"Vecerek","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Transcriptional profiling of \u003ci\u003eBordetella pertussis\u003c/i\u003e reveals requirement of RNA chaperone Hfq for Type III secretion system functionality"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Computational Biology"},{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Chemistry"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-04-14","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1017237","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["374047 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eBordetella pertussis\u003c/i\u003e, the causative agent of human whooping cough (pertussis) produces a complex array of virulence factors in order to establish efficient infection in the host. The RNA chaperone Hfq and small regulatory RNAs are key players in posttranscriptional regulation in bacteria and have been shown to play an essential role in virulence of a broad spectrum of bacterial pathogens. This study represents the first attempt to characterize the Hfq regulon of the human pathogen \u003ci\u003eB. pertussis\u003c/i\u003e under laboratory conditions as well as upon passage in the host and indicates that loss of Hfq has a profound effect on gene expression in \u003ci\u003eB. pertussis\u003c/i\u003e. Comparative transcriptional profiling revealed that Hfq is required for expression of several virulence factors in \u003ci\u003eB. pertussis\u003c/i\u003e cells including the Type III secretion system (T3SS). In striking contrast to the wt strain, T3SS did not become operational in the \u003ci\u003ehfq\u003c/i\u003e mutant passaged either through mice or macrophages thereby proving that Hfq is required for the functionality of the \u003ci\u003eB. pertussis\u003c/i\u003e T3SS. Likewise, expression of virulence factors \u003ci\u003evag8\u003c/i\u003e and \u003ci\u003etcfA\u003c/i\u003e encoding autotransporter and tracheal colonization factor, respectively, was strongly reduced in the \u003ci\u003ehfq\u003c/i\u003e mutant. Importantly, for the first time we demonstrate that \u003ci\u003eB. pertussis\u003c/i\u003e T3SS can be activated upon contact with macrophage cells \u003ci\u003ein vitro\u003c/i\u003e.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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