{"data":{"id":"10.6084/m9.figshare.1378745.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1378745.v3","prefix":"10.6084","suffix":"m9.figshare.1378745.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Dharmesh Sabharwal","affiliation":[],"nameIdentifiers":[]},{"name":"Tianyan Song","affiliation":[],"nameIdentifiers":[]},{"name":"Papenfort, Kai","nameType":"Personal","givenName":"Kai","familyName":"Papenfort","affiliation":[],"nameIdentifiers":[]},{"name":"Wai, Sun Nyunt","nameType":"Personal","givenName":"Sun Nyunt","familyName":"Wai","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The VrrA sRNA controls a stationary phase survival factor Vrp of \u003ci\u003eVibrio cholerae\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-15","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1017211","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1378745","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1855280 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Small non-coding RNAs (sRNAs) are emerging regulatory elements in bacteria. The \u003ci\u003eVibrio cholerae\u003c/i\u003e sRNA VrrA has previously been shown to down-regulate outer membrane proteins (OmpA and OmpT) and biofilm matrix protein (RbmC) by base-pairing with the 5′ region of the corresponding mRNAs. In this study, we present an additional target of VrrA in \u003ci\u003eV. cholerae\u003c/i\u003e, the mRNA coding for the ribosome binding protein Vrp. Vrp is homologous to ribosome-associated inhibitor A (RaiA) of \u003ci\u003eEscherichia coli\u003c/i\u003e which facilitates stationary phase survival through ribosome hibernation. We show that VrrA down-regulates Vrp protein synthesis by base-pairing to the 5′ region of \u003ci\u003evrp\u003c/i\u003e mRNA and that the regulation requires the RNA chaperone protein, Hfq. We further demonstrate that Vrp is highly expressed during stationary phase growth and associates with the ribosome of \u003ci\u003eV. cholerae\u003c/i\u003e. The effect of the Vrp protein in starvation survival is synergistic with that of the VC2530 protein, a homolog of the \u003ci\u003eE. coli\u003c/i\u003e hibernation promoting factor HPF, suggesting a combined role for these proteins in ribosome hibernation in \u003ci\u003eV. cholerae\u003c/i\u003e. Vrp and VC2530 are important for \u003ci\u003eV. cholerae\u003c/i\u003e starvation survival under nutrient deficient conditions. While VC2530 is down-regulated in cells lacking \u003ci\u003evrrA\u003c/i\u003e, mutation of \u003ci\u003evrp\u003c/i\u003e results in VC2530 activation. This is the first report indicating a regulatory role for an sRNA, modulating stationary factors involved in bacterial ribosome hibernation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjEzNzg3NDUuVjM8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5EaGFybWVzaCBTYWJoYXJ3YWw8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5UaWFueWFuIFNvbmc8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5LYWkgUGFwZW5mb3J0PC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+U3VuIE55dW50IFdhaTwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgPC9jcmVhdG9ycz4KICA8dGl0bGVzPgogICAgPHRpdGxlPlRoZSBWcnJBIHNSTkEgY29udHJvbHMgYSBzdGF0aW9uYXJ5IHBoYXNlIHN1cnZpdmFsIGZhY3RvciBWcnAgb2YgJmx0O2kmZ3Q7VmlicmlvIGNob2xlcmFlJmx0Oy9pJmd0OzwvdGl0bGU+CiAgPC90aXRsZXM+CiAgPGRlc2NyaXB0aW9ucz4KICAgIDxkZXNjcmlwdGlvbiBkZXNjcmlwdGlvblR5cGU9IkFic3RyYWN0Ij4mbHQ7ZGl2Jmd0OyZsdDtwJmd0O1NtYWxsIG5vbi1jb2RpbmcgUk5BcyAoc1JOQXMpIGFyZSBlbWVyZ2luZyByZWd1bGF0b3J5IGVsZW1lbnRzIGluIGJhY3RlcmlhLiBUaGUgJmx0O2kmZ3Q7VmlicmlvIGNob2xlcmFlJmx0Oy9pJmd0OyBzUk5BIFZyckEgaGFzIHByZXZpb3VzbHkgYmVlbiBzaG93biB0byBkb3duLXJlZ3VsYXRlIG91dGVyIG1lbWJyYW5lIHByb3RlaW5zIChPbXBBIGFuZCBPbXBUKSBhbmQgYmlvZmlsbSBtYXRyaXggcHJvdGVpbiAoUmJtQykgYnkgYmFzZS1wYWlyaW5nIHdpdGggdGhlIDXigLIgcmVnaW9uIG9mIHRoZSBjb3JyZXNwb25kaW5nIG1STkFzLiBJbiB0aGlzIHN0dWR5LCB3ZSBwcmVzZW50IGFuIGFkZGl0aW9uYWwgdGFyZ2V0IG9mIFZyckEgaW4gJmx0O2kmZ3Q7Vi4gY2hvbGVyYWUmbHQ7L2kmZ3Q7LCB0aGUgbVJOQSBjb2RpbmcgZm9yIHRoZSByaWJvc29tZSBiaW5kaW5nIHByb3RlaW4gVnJwLiBWcnAgaXMgaG9tb2xvZ291cyB0byByaWJvc29tZS1hc3NvY2lhdGVkIGluaGliaXRvciBBIChSYWlBKSBvZiAmbHQ7aSZndDtFc2NoZXJpY2hpYSBjb2xpJmx0Oy9pJmd0OyB3aGljaCBmYWNpbGl0YXRlcyBzdGF0aW9uYXJ5IHBoYXNlIHN1cnZpdmFsIHRocm91Z2ggcmlib3NvbWUgaGliZXJuYXRpb24uIFdlIHNob3cgdGhhdCBWcnJBIGRvd24tcmVndWxhdGVzIFZycCBwcm90ZWluIHN5bnRoZXNpcyBieSBiYXNlLXBhaXJpbmcgdG8gdGhlIDXigLIgcmVnaW9uIG9mICZsdDtpJmd0O3ZycCZsdDsvaSZndDsgbVJOQSBhbmQgdGhhdCB0aGUgcmVndWxhdGlvbiByZXF1aXJlcyB0aGUgUk5BIGNoYXBlcm9uZSBwcm90ZWluLCBIZnEuIFdlIGZ1cnRoZXIgZGVtb25zdHJhdGUgdGhhdCBWcnAgaXMgaGlnaGx5IGV4cHJlc3NlZCBkdXJpbmcgc3RhdGlvbmFyeSBwaGFzZSBncm93dGggYW5kIGFzc29jaWF0ZXMgd2l0aCB0aGUgcmlib3NvbWUgb2YgJmx0O2kmZ3Q7Vi4gY2hvbGVyYWUmbHQ7L2kmZ3Q7LiBUaGUgZWZmZWN0IG9mIHRoZSBWcnAgcHJvdGVpbiBpbiBzdGFydmF0aW9uIHN1cnZpdmFsIGlzIHN5bmVyZ2lzdGljIHdpdGggdGhhdCBvZiB0aGUgVkMyNTMwIHByb3RlaW4sIGEgaG9tb2xvZyBvZiB0aGUgJmx0O2kmZ3Q7RS4gY29saSZsdDsvaSZndDsgaGliZXJuYXRpb24gcHJvbW90aW5nIGZhY3RvciBIUEYsIHN1Z2dlc3RpbmcgYSBjb21iaW5lZCByb2xlIGZvciB0aGVzZSBwcm90ZWlucyBpbiByaWJvc29tZSBoaWJlcm5hdGlvbiBpbiAmbHQ7aSZndDtWLiBjaG9sZXJhZSZsdDsvaSZndDsuIFZycCBhbmQgVkMyNTMwIGFyZSBpbXBvcnRhbnQgZm9yICZsdDtpJmd0O1YuIGNob2xlcmFlJmx0Oy9pJmd0OyBzdGFydmF0aW9uIHN1cnZpdmFsIHVuZGVyIG51dHJpZW50IGRlZmljaWVudCBjb25kaXRpb25zLiBXaGlsZSBWQzI1MzAgaXMgZG93bi1yZWd1bGF0ZWQgaW4gY2VsbHMgbGFja2luZyAmbHQ7aSZndDt2cnJBJmx0Oy9pJmd0OywgbXV0YXRpb24gb2YgJmx0O2kmZ3Q7dnJwJmx0Oy9pJmd0OyByZXN1bHRzIGluIFZDMjUzMCBhY3RpdmF0aW9uLiBUaGlzIGlzIHRoZSBmaXJzdCByZXBvcnQgaW5kaWNhdGluZyBhIHJlZ3VsYXRvcnkgcm9sZSBmb3IgYW4gc1JOQSwgbW9kdWxhdGluZyBzdGF0aW9uYXJ5IGZhY3RvcnMgaW52b2x2ZWQgaW4gYmFjdGVyaWFsIHJpYm9zb21lIGhpYmVybmF0aW9uLiZsdDsvcCZndDsmbHQ7L2RpdiZndDs8L2Rlc2NyaXB0aW9uPgogIDwvZGVzY3JpcHRpb25zPgogIDxzdWJqZWN0cz4KICAgIDxzdWJqZWN0IHNjaGVtZVVSST0iaHR0cDovL3d3dy5hYnMuZ292LmF1L2F1c3N0YXRzL2Fic0AubnNmLzAvNkJCNDI3QUI5Njk2QzIyNUNBMjU3NDE4MDAwNDQ2M0UiIHN1YmplY3RTY2hlbWU9IkZPUiI+MTEwMzA5IEluZmVjdGlvdXMgRGlzZWFzZXM8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5Jbm9yZ2FuaWMgQ2hlbWlzdHJ5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+Q2FuY2VyPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvbG9naWNhbCBTY2llbmNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkltbXVub2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5FYXJ0aCBhbmQgRW52aXJvbm1lbnRhbCBTY2llbmNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkJpb3RlY2hub2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5Nb2xlY3VsYXIgQmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkdlbmV0aWNzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+Q2VsbCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+TWljcm9iaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvY2hlbWlzdHJ5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvcGh5c2ljczwvc3ViamVjdD4KICA8L3N1YmplY3RzPgogIDxwdWJsaXNoZXI+VGF5bG9yICZhbXA7IEZyYW5jaXM8L3B1Ymxpc2hlcj4KICA8cHVibGljYXRpb25ZZWFyPjIwMTU8L3B1YmxpY2F0aW9uWWVhcj4KICA8ZGF0ZXM+CiAgICA8ZGF0ZSBkYXRlVHlwZT0iQ3JlYXRlZCI+MjAxNS0xMC0xNTwvZGF0ZT4KICAgIDxkYXRlIGRhdGVUeXBlPSJVcGRhdGVkIj4yMDE2LTAxLTE5PC9kYXRlPgogIDwvZGF0ZXM+CiAgPHJlc291cmNlVHlwZSByZXNvdXJjZVR5cGVHZW5lcmFsPSJEYXRhc2V0Ij5EYXRhc2V0PC9yZXNvdXJjZVR5cGU+CiAgPHNpemVzPgogICAgPHNpemU+MTg1NTI4MCBCeXRlczwvc2l6ZT4KICA8L3NpemVzPgogIDxyZWxhdGVkSWRlbnRpZmllcnM+CiAgICA8cmVsYXRlZElkZW50aWZpZXIgcmVsYXRlZElkZW50aWZpZXJUeXBlPSJET0kiIHJlbGF0aW9uVHlwZT0iSXNTdXBwbGVtZW50VG8iPjEwLjEwODAvMTU0NzY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