{"data":{"id":"10.6084/m9.figshare.13292912.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.13292912.v1","prefix":"10.6084","suffix":"m9.figshare.13292912.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"YuQian, Wu","nameType":"Personal","givenName":"Wu","familyName":"YuQian","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Large-Scale Isolation and Antitumor Mechanism Evaluation of Compounds from the Traditional Chinese Medicine Cordyceps Militaris"}],"publisher":"figshare","container":{},"publicationYear":2020,"subjects":[{"subject":"30401 Biologically Active Molecules","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"NMR Spectroscopy"},{"subject":"30405 Molecular Medicine","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2020-11-26","dateType":"Created"},{"date":"2020-11-26","dateType":"Updated"},{"date":"2020","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.13292912","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["82401074 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"We established a large-scale separation and purification platform to obtain kilogram amounts of natural compounds from the extraction of the fruiting bodies of \u003ci\u003eC. militaris. \u003c/i\u003eSeven monomeric compounds, N6-(2-hydroxyethyl) adenosine (HEA), ergosterol (E), ergosta-7,22-diene-3,5,6-triol (EI), 5α,8α-epidioxy-(22\u003ci\u003eE\u003c/i\u003e,24\u003ci\u003eR\u003c/i\u003e)-ergosta-6,22-dien-3β-ol (ED), ergosta-7,22-dien-3β,5α-dihydroxy-6-one(EO),(20\u003ci\u003eS\u003c/i\u003e,22\u003ci\u003eE\u003c/i\u003e,24\u003ci\u003eR\u003c/i\u003e)-Eegosta-7,22-dien-3β,5α,6β,9α-tetraol22\u003ci\u003eE\u003c/i\u003e,24\u003ci\u003eR\u003c/i\u003e-ergosta-7,22-diene-3β,5α,6β,9α-tetraol (ET), and (24\u003ci\u003eS\u003c/i\u003e)-5,22-stigmastadien-3β-ol (SE), were harvested using different solvents, and the structure of each compound was identified. The activities and functions of the isolated compounds were tested by label-free, real-time cell analysis methods at the cellular level, and their antitumor effects were verified using mouse models of Lewis and H22 tumors. The anti-insomnia effect of HEAadenosine was tested in an anti-insomnia mouse model. The interactions between E and 8 A549 cell proteins were determined. The biosynthetic pathways of HEA and E, which possess pharmacologically active monomers, were determined. This platform can provide a theoretical basis for the further development and discovery of novel natural medicines.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/Large-Scale_Isolation_and_Antitumor_Mechanism_Evaluation_of_Compounds_from_the_Traditional_Chinese_Medicine_Cordyceps_Militaris/13292912/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2020-11-26T14:59:26.000Z","registered":"2020-11-26T14:59:27.000Z","published":"2020","updated":"2020-11-26T15:08:06.000Z"},"relationships":{"client":{"data":{"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