{"data":{"id":"10.6084/m9.figshare.13292912","type":"dois","attributes":{"doi":"10.6084/m9.figshare.13292912","prefix":"10.6084","suffix":"m9.figshare.13292912","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"YuQian, Wu","nameType":"Personal","givenName":"Wu","familyName":"YuQian","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Antitumor Compounds from Cordyceps Militaris"}],"publisher":"figshare","container":{},"publicationYear":2020,"subjects":[{"subject":"30401 Biologically Active Molecules","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"NMR Spectroscopy"},{"subject":"30405 Molecular Medicine","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2020-11-26","dateType":"Created"},{"date":"2020-11-26","dateType":"Updated"},{"date":"2020","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["82401074 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"We established a large-scale separation and purification platform to obtain kilogram amounts of natural compounds from the extraction of the fruiting bodies of \u003ci\u003eC. militaris. \u003c/i\u003eSeven monomeric compounds, N6-(2-hydroxyethyl) adenosine (HEA), ergosterol (E), ergosta-7,22-diene-3,5,6-triol (EI), 5α,8α-epidioxy-(22\u003ci\u003eE\u003c/i\u003e,24\u003ci\u003eR\u003c/i\u003e)-ergosta-6,22-dien-3β-ol (ED), ergosta-7,22-dien-3β,5α-dihydroxy-6-one(EO),(20\u003ci\u003eS\u003c/i\u003e,22\u003ci\u003eE\u003c/i\u003e,24\u003ci\u003eR\u003c/i\u003e)-Eegosta-7,22-dien-3β,5α,6β,9α-tetraol22\u003ci\u003eE\u003c/i\u003e,24\u003ci\u003eR\u003c/i\u003e-ergosta-7,22-diene-3β,5α,6β,9α-tetraol (ET), and (24\u003ci\u003eS\u003c/i\u003e)-5,22-stigmastadien-3β-ol (SE), were harvested using different solvents, and the structure of each compound was identified. The activities and functions of the isolated compounds were tested by label-free, real-time cell analysis methods at the cellular level, and their antitumor effects were verified using mouse models of Lewis and H22 tumors. The anti-insomnia effect of HEAadenosine was tested in an anti-insomnia mouse model. The interactions between E and 8 A549 cell proteins were determined. The biosynthetic pathways of HEA and E, which possess pharmacologically active monomers, were determined. This platform can provide a theoretical basis for the further development and discovery of novel natural medicines.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/Large-Scale_Isolation_and_Antitumor_Mechanism_Evaluation_of_Compounds_from_the_Traditional_Chinese_Medicine_Cordyceps_Militaris/13292912","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2020-11-26T14:59:26.000Z","registered":"2020-11-26T14:59:27.000Z","published":"2020","updated":"2020-11-26T15:08:01.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.13292912","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}