{"data":{"id":"10.6084/m9.figshare.1325063.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1325063.v2","prefix":"10.6084","suffix":"m9.figshare.1325063.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Filo, Julie","nameType":"Personal","givenName":"Julie","familyName":"Filo","affiliation":[],"nameIdentifiers":[]},{"name":"Wu, Austin","nameType":"Personal","givenName":"Austin","familyName":"Wu","affiliation":[],"nameIdentifiers":[]},{"name":"Eliason, Erica","nameType":"Personal","givenName":"Erica","familyName":"Eliason","affiliation":[],"nameIdentifiers":[]},{"name":"Richardson, Timothy","nameType":"Personal","givenName":"Timothy","familyName":"Richardson","affiliation":[],"nameIdentifiers":[]},{"name":"Thines, Bryan C","nameType":"Personal","givenName":"Bryan C","familyName":"Thines","affiliation":[],"nameIdentifiers":[]},{"name":"Harmon, Frank G","nameType":"Personal","givenName":"Frank G","familyName":"Harmon","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Gibberellin driven growth in \u003ci\u003eelf3\u003c/i\u003e mutants requires PIF4 and PIF5"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Computational Biology"},{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-04-21","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15592324.2014.992707","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1325063","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3489959 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The regulatory connections between the circadian clock and hormone signaling are essential to understand, as these two regulatory processes work together to time growth processes relative to predictable environmental events. Gibberellins (GAs) are phytohormones that control many growth processes throughout all stages of the plant life cycle, including germination and flowering. An increasing number of examples demonstrate that the circadian clock directly influences GA biosynthesis and signaling. EARLY FLOWERING 3 (ELF3) participates in a tripartite transcriptional complex known as the Evening Complex (EC). In this capacity, ELF3 is fundamental to core circadian clock activity, as well as time-of-day specific regulation of genes directly responsible for growth control, namely the \u003ci\u003ePHYTOCHROME INTERACTING FACTOR 4\u003c/i\u003e (\u003ci\u003ePIF4\u003c/i\u003e) and \u003ci\u003ePIF5\u003c/i\u003e genes. Here we show that the GA biosynthesis inhibitor paclobutrazol substantially reduces the long hypocotyl and petiole phenotypes of Arabidopsis \u003ci\u003eelf3\u003c/i\u003e mutants. In addition, loss of ELF3 activity causes upregulation of the key GA biosynthesis genes \u003ci\u003eGA20ox1\u003c/i\u003e and \u003ci\u003eGA20ox2\u003c/i\u003e. Moreover, \u003ci\u003eGA20ox1\u003c/i\u003e and \u003ci\u003eGA20ox2\u003c/i\u003e expression depends strongly on the redundant activities of \u003ci\u003ePIF4\u003c/i\u003e and \u003ci\u003ePIF5\u003c/i\u003e. These findings indicate that the defining growth phenotypes of \u003ci\u003eelf3\u003c/i\u003e mutants arise from altered GA biosynthesis due to misregulation of \u003ci\u003ePIF4\u003c/i\u003e and \u003ci\u003ePIF5\u003c/i\u003e. These observations agree with recent work linking increased GA production with the elongated growth phenotypes of the barley \u003ci\u003eelf3\u003c/i\u003e mutant. Thus, the role of the EC in regulation of GA biosynthesis and signaling in eudicots is shared with monocots and, therefore, is a highly conserved mechanism for growth control.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Gibberellin_driven_growth_in_i_elf3_i_mutants_requires_PIF4_and_PIF5/1325063/2","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T15:51:48.000Z","registered":"2016-01-19T15:51:49.000Z","published":"2015","updated":"2020-09-04T18:44:38.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1325063.v2","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}