{"data":{"id":"10.6084/m9.figshare.1312986.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1312986.v2","prefix":"10.6084","suffix":"m9.figshare.1312986.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Imai, Hiroe","nameType":"Personal","givenName":"Hiroe","familyName":"Imai","affiliation":[],"nameIdentifiers":[]},{"name":"Noda, Yusaku","nameType":"Personal","givenName":"Yusaku","familyName":"Noda","affiliation":[],"nameIdentifiers":[]},{"name":"Tamaoki, Masanori","nameType":"Personal","givenName":"Masanori","familyName":"Tamaoki","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Alteration of \u003ci\u003eArabidopsis SLAC1\u003c/i\u003e promoter and its association with natural variation in drought tolerance"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Physics"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-04-27","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15592324.2014.989761","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1312986","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["547683 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Natural variation for drought tolerance is a major issue in adaptation and geographic distribution of terrestrial plants. Despite the importance, little is known about the genes and molecular mechanisms that determine its naturally occurring diversity. We analyzed the intraspecific drought tolerance variation between 2 accessions of \u003ci\u003eArabidopsis thaliana\u003c/i\u003e, Columbia (Col)-0 and Wassilewskija (Ws)-2. Measurement of weight loss in detached seedlings demonstrated a clear difference between drought-tolerant Col-0 and drought-sensitive Ws-2. They also differed in their stomatal response under drought condition. Using a quantitative genetic approach, we found a significant quantitative locus on chromosome 1. Surveying in the locus, we extrapolated that the \u003ci\u003eSLAC1\u003c/i\u003e gene, which is associated with stomatal closure, was likely responsible for the difference of drought tolerance. Comparison of their nucleotide and amino acid sequences revealed that there were few differences in regions encoding \u003ci\u003eSLAC1\u003c/i\u003e protein but was a large deletion in \u003ci\u003eSLAC1\u003c/i\u003e promoter of Ws-2. Histochemical GUS staining showed that the \u003ci\u003eSLAC1\u003c/i\u003e expressed dominantly in guard cells of Col-0, but did less in guard cells of Ws-2. Quantitative PCR analysis also showed that transcript level of \u003ci\u003eSLAC1\u003c/i\u003e in guard cells was higher in Col-0 than in Ws-2. The \u003ci\u003eSLAC1\u003c/i\u003e transcription analyses indicate low accumulation of \u003ci\u003eSLAC1\u003c/i\u003e in guard cells of Ws-2. When taken together, our results suggest that the low drought tolerance of Ws-2 was associated with the deletion of the promoter region of Ws-2 \u003ci\u003eSLAC1\u003c/i\u003e.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjEzMTI5ODYuVjI8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5IaXJvZSBJbWFpPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+WXVzYWt1IE5vZGE8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5NYXNhbm9yaSBUYW1hb2tpPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICA8L2NyZWF0b3JzPgogIDx0aXRsZXM+CiAgICA8dGl0bGU+QWx0ZXJhdGlvbiBvZiAmbHQ7aSZndDtBcmFiaWRvcHNpcyBTTEFDMSZsdDsvaSZndDsgcHJvbW90ZXIgYW5kIGl0cyBhc3NvY2lhdGlvbiB3aXRoIG5hdHVyYWwgdmFyaWF0aW9uIGluIGRyb3VnaHQgdG9sZXJhbmNlPC90aXRsZT4KICA8L3RpdGxlcz4KICA8ZGVzY3JpcHRpb25zPgogICAgPGRlc2NyaXB0aW9uIGRlc2NyaXB0aW9uVHlwZT0iQWJzdHJhY3QiPiZsdDtkaXYmZ3Q7Jmx0O3AmZ3Q7TmF0dXJhbCB2YXJpYXRpb24gZm9yIGRyb3VnaHQgdG9sZXJhbmNlIGlzIGEgbWFqb3IgaXNzdWUgaW4gYWRhcHRhdGlvbiBhbmQgZ2VvZ3JhcGhpYyBkaXN0cmlidXRpb24gb2YgdGVycmVzdHJpYWwgcGxhbnRzLiBEZXNwaXRlIHRoZSBpbXBvcnRhbmNlLCBsaXR0bGUgaXMga25vd24gYWJvdXQgdGhlIGdlbmVzIGFuZCBtb2xlY3VsYXIgbWVjaGFuaXNtcyB0aGF0IGRldGVybWluZSBpdHMgbmF0dXJhbGx5IG9jY3VycmluZyBkaXZlcnNpdHkuIFdlIGFuYWx5emVkIHRoZSBpbnRyYXNwZWNpZmljIGRyb3VnaHQgdG9sZXJhbmNlIHZhcmlhdGlvbiBiZXR3ZWVuIDIgYWNjZXNzaW9ucyBvZiAmbHQ7aSZndDtBcmFiaWRvcHNpcyB0aGFsaWFuYSZsdDsvaSZndDssIENvbHVtYmlhIChDb2wpLTAgYW5kIFdhc3NpbGV3c2tpamEgKFdzKS0yLiBNZWFzdXJlbWVudCBvZiB3ZWlnaHQgbG9zcyBpbiBkZXRhY2hlZCBzZWVkbGluZ3MgZGVtb25zdHJhdGVkIGEgY2xlYXIgZGlmZmVyZW5jZSBiZXR3ZWVuIGRyb3VnaHQtdG9sZXJhbnQgQ29sLTAgYW5kIGRyb3VnaHQtc2Vuc2l0aXZlIFdzLTIuIFRoZXkgYWxzbyBkaWZmZXJlZCBpbiB0aGVpciBzdG9tYXRhbCByZXNwb25zZSB1bmRlciBkcm91Z2h0IGNvbmRpdGlvbi4gVXNpbmcgYSBxdWFudGl0YXRpdmUgZ2VuZXRpYyBhcHByb2FjaCwgd2UgZm91bmQgYSBzaWduaWZpY2FudCBxdWFudGl0YXRpdmUgbG9jdXMgb24gY2hyb21vc29tZSAxLiBTdXJ2ZXlpbmcgaW4gdGhlIGxvY3VzLCB3ZSBleHRyYXBvbGF0ZWQgdGhhdCB0aGUgJmx0O2kmZ3Q7U0xBQzEmbHQ7L2kmZ3Q7IGdlbmUsIHdoaWNoIGlzIGFzc29jaWF0ZWQgd2l0aCBzdG9tYXRhbCBjbG9zdXJlLCB3YXMgbGlrZWx5IHJlc3BvbnNpYmxlIGZvciB0aGUgZGlmZmVyZW5jZSBvZiBkcm91Z2h0IHRvbGVyYW5jZS4gQ29tcGFyaXNvbiBvZiB0aGVpciBudWNsZW90aWRlIGFuZCBhbWlubyBhY2lkIHNlcXVlbmNlcyByZXZlYWxlZCB0aGF0IHRoZXJlIHdlcmUgZmV3IGRpZmZlcmVuY2VzIGluIHJlZ2lvbnMgZW5jb2RpbmcgJmx0O2kmZ3Q7U0xBQzEmbHQ7L2kmZ3Q7IHByb3RlaW4gYnV0IHdhcyBhIGxhcmdlIGRlbGV0aW9uIGluICZsdDtpJmd0O1NMQUMxJmx0Oy9pJmd0OyBwcm9tb3RlciBvZiBXcy0yLiBIaXN0b2NoZW1pY2FsIEdVUyBzdGFpbmluZyBzaG93ZWQgdGhhdCB0aGUgJmx0O2kmZ3Q7U0xBQzEmbHQ7L2kmZ3Q7IGV4cHJlc3NlZCBkb21pbmFudGx5IGluIGd1YXJkIGNlbGxzIG9mIENvbC0wLCBidXQgZGlkIGxlc3MgaW4gZ3VhcmQgY2VsbHMgb2YgV3MtMi4gUXVhbnRpdGF0aXZlIFBDUiBhbmFseXNpcyBhbHNvIHNob3dlZCB0aGF0IHRyYW5zY3JpcHQgbGV2ZWwgb2YgJmx0O2kmZ3Q7U0xBQzEmbHQ7L2kmZ3Q7IGluIGd1YXJkIGNlbGxzIHdhcyBoaWdoZXIgaW4gQ29sLTAgdGhhbiBpbiBXcy0yLiBUaGUgJmx0O2kmZ3Q7U0xBQzEmbHQ7L2kmZ3Q7IHRyYW5zY3JpcHRpb24gYW5hbHlzZXMgaW5kaWNhdGUgbG93IGFjY3VtdWxhdGlvbiBvZiAmbHQ7aSZndDtTTEFDMSZsdDsvaSZndDsgaW4gZ3VhcmQgY2VsbHMgb2YgV3MtMi4gV2hlbiB0YWtlbiB0b2dldGhlciwgb3VyIHJlc3VsdHMgc3VnZ2VzdCB0aGF0IHRoZSBsb3cgZHJvdWdodCB0b2xlcmFuY2Ugb2YgV3MtMiB3YXMgYXNzb2NpYXRlZCB3aXRoIHRoZSBkZWxldGlvbiBvZiB0aGUgcHJvbW90ZXIgcmVnaW9uIG9mIFdzLTIgJmx0O2kmZ3Q7U0xBQzEmbHQ7L2kmZ3Q7LiZsdDsvcCZndDsmbHQ7L2RpdiZndDs8L2Rlc2NyaXB0aW9uPgogIDwvZGVzY3JpcHRpb25zPgogIDxzdWJqZWN0cz4KICAgIDxzdWJqZWN0IHNjaGVtZVVSST0iaHR0cDovL3d3dy5hYnMuZ292LmF1L2F1c3N0YXRzL2Fic0AubnNmLzAvNkJCNDI3QUI5Njk2QzIyNUNBMjU3NDE4MDAwNDQ2M0UiIHN1YmplY3RTY2hlbWU9IkZPUiI+NjA1MDYgVmlyb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5QbGFudCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc2NoZW1lVVJJPSJodHRwOi8vd3d3LmFicy5nb3YuYXUvYXVzc3RhdHMvYWJzQC5uc2YvMC82QkI0MjdBQjk2OTZDMjI1Q0EyNTc0MTgwMDA0NDYzRSIgc3ViamVjdFNjaGVtZT0iRk9SIj4xMTAzMDkgSW5mZWN0aW91cyBEaXNlYXNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkJpb2xvZ2ljYWwgU2NpZW5jZXM8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5JbW11bm9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RWNvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkVhcnRoIGFuZCBFbnZpcm9ubWVudGFsIFNjaWVuY2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+TW9sZWN1bGFyIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5HZW5ldGljczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkNlbGwgQmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PlBoeXNpY3M8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9waHlzaWNzPC9zdWJqZWN0PgogIDwvc3ViamVjdHM+CiAgPHB1Ymxpc2hlcj5UYXlsb3IgJmFtcDsgRnJhbmNpczwvcHVibGlzaGVyPgogIDxwdWJsaWNhdGlvblllYXI+MjAxNTwvcHVibGljYXRpb25ZZWFyPgogIDxkYXRlcz4KICAgIDxkYXRlIGRhdGVUeXBlPSJDcmVhdGVkIj4yMDE1LTA0LTI3PC9kYXRlPgogICAgPGRhdGUgZGF0ZVR5cGU9IlVwZGF0ZWQiPjIwMTYtMDEtMTk8L2RhdGU+CiAgPC9kYXRlcz4KICA8cmVzb3VyY2VUeXBlIHJlc291cmNlVHlwZUdlbmVyYWw9IkRhdGFzZXQiPkRhdGFzZXQ8L3Jlc291cmNlVHlwZT4KICA8c2l6ZXM+CiAgICA8c2l6ZT41NDc2ODMgQnl0ZXM8L3NpemU+CiAgPC9zaXplcz4KICA8cmVsYXRlZElkZW50aWZpZX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