{"data":{"id":"10.6084/m9.figshare.1310468.v4","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1310468.v4","prefix":"10.6084","suffix":"m9.figshare.1310468.v4","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Nishiyama, Keita","nameType":"Personal","givenName":"Keita","familyName":"Nishiyama","affiliation":[],"nameIdentifiers":[]},{"name":"Nakamata, Koichi","nameType":"Personal","givenName":"Koichi","familyName":"Nakamata","affiliation":[],"nameIdentifiers":[]},{"name":"Ueno, Shintaro","nameType":"Personal","givenName":"Shintaro","familyName":"Ueno","affiliation":[],"nameIdentifiers":[]},{"name":"Terao, Akari","nameType":"Personal","givenName":"Akari","familyName":"Terao","affiliation":[],"nameIdentifiers":[]},{"name":"Aryantini, Ni Putu Desy","nameType":"Personal","givenName":"Ni Putu Desy","familyName":"Aryantini","affiliation":[],"nameIdentifiers":[]},{"name":"I. Nengah Sujaya","affiliation":[],"nameIdentifiers":[]},{"name":"Fukuda, Kenji","nameType":"Personal","givenName":"Kenji","familyName":"Fukuda","affiliation":[],"nameIdentifiers":[]},{"name":"Urashima, Tadasu","nameType":"Personal","givenName":"Tadasu","familyName":"Urashima","affiliation":[],"nameIdentifiers":[]},{"name":"Yamamoto, Yuji","nameType":"Personal","givenName":"Yuji","familyName":"Yamamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Mukai, Takao","nameType":"Personal","givenName":"Takao","familyName":"Mukai","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Adhesion properties of \u003ci\u003eLactobacillus rhamnosus\u003c/i\u003e mucus-binding factor to mucin and extracellular matrix proteins"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Evolutionary Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"Physiology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Physics"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2014.972325","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1310468","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1472957 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"We previously described potential probiotic \u003ci\u003eLactobacillus rhamnosus\u003c/i\u003e strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of \u003ci\u003eL. rhamnosus\u003c/i\u003e as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of \u003ci\u003eL. rhamnosus\u003c/i\u003e from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 \u003ci\u003embf\u003c/i\u003e mutant strain to PCM and ECM proteins was significantly less than that of the wild-type strain. Collectively, these results suggested that MBF contribute to \u003ci\u003eL. rhamnosus\u003c/i\u003e host colonization via mucin and ECM protein binding.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Adhesion_properties_of_i_Lactobacillus_rhamnosus_i_mucus_binding_factor_to_mucin_and_extracellular_matrix_proteins/1310468/4","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T15:16:22.000Z","registered":"2016-01-19T15:16:23.000Z","published":"2015","updated":"2020-09-04T18:57:50.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1310468.v4","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}