{"data":{"id":"10.6084/m9.figshare.1310467","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1310467","prefix":"10.6084","suffix":"m9.figshare.1310467","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Katayama, Takuya","nameType":"Personal","givenName":"Takuya","familyName":"Katayama","affiliation":[],"nameIdentifiers":[]},{"name":"Ohta, Akinori","nameType":"Personal","givenName":"Akinori","familyName":"Ohta","affiliation":[],"nameIdentifiers":[]},{"name":"Horiuchi, Hiroyuki","nameType":"Personal","givenName":"Hiroyuki","familyName":"Horiuchi","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Protein kinase C regulates the expression of cell wall-related genes in RlmA-dependent and independent manners in \u003ci\u003eAspergillus nidulans\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Computational Biology"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-02-17","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2014.973365","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["640306 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"A protein kinase C of \u003ci\u003eAspergillus nidulans\u003c/i\u003e, PkcA, is required for cell wall integrity (CWI) and is considered a major component of the regulating pathway. To investigate whether PkcA regulates the transcription of cell wall-related genes, we constructed strains expressing \u003ci\u003epkcA\u003c/i\u003e(R429A) that encodes an activated form of PkcA. The mRNA levels of most chitin synthase genes and an α-glucan synthase gene, \u003ci\u003eagsB\u003c/i\u003e, were increased when \u003ci\u003epkcA\u003c/i\u003e(R429A) expression was induced. These mRNA increases were not observed or were only partially observed, in a deletion mutant of \u003ci\u003erlmA\u003c/i\u003e, an ortholog of \u003ci\u003eRLM1\u003c/i\u003e that encodes a transcription factor in the CWI pathway in \u003ci\u003eSaccharomyces cerevisiae\u003c/i\u003e. In addition, in a \u003ci\u003epkcA\u003c/i\u003e temperature-sensitive mutant under heat stress, the mRNA levels of some chitin synthase genes and \u003ci\u003eagsB\u003c/i\u003e did not increase. These results suggest that PkcA is involved in CWI maintenance through the transcriptional regulation of cell wall-related genes.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Protein_kinase_C_regulates_the_expression_of_cell_wall_related_genes_in_RlmA_dependent_and_independent_manners_in_i_Aspergillus_nidulans_i_/1310467","contentUrl":null,"metadataVersion":5,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-02-17T11:05:14.000Z","registered":"2015-02-17T11:05:15.000Z","published":"2015","updated":"2020-09-04T18:57:42.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"pr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