{"data":{"id":"10.6084/m9.figshare.1310466","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1310466","prefix":"10.6084","suffix":"m9.figshare.1310466","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Takahashi, Shigekazu","nameType":"Personal","givenName":"Shigekazu","familyName":"Takahashi","affiliation":[],"nameIdentifiers":[]},{"name":"Abe, Eriko","nameType":"Personal","givenName":"Eriko","familyName":"Abe","affiliation":[],"nameIdentifiers":[]},{"name":"Nakayama, Katsumi","nameType":"Personal","givenName":"Katsumi","familyName":"Nakayama","affiliation":[],"nameIdentifiers":[]},{"name":"Satoh, Hiroyuki","nameType":"Personal","givenName":"Hiroyuki","familyName":"Satoh","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Identification of genes encoding photoconvertible (Class I) water-soluble chlorophyll-binding proteins from \u003ci\u003eChenopodium ficifolium\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Ecology"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-02-17","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2014.972326","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["483752 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Photoconvertible water-soluble chlorophyll-binding proteins, called Class I WSCPs, have been detected in Chenopodiaceae, Amaranthaceae and Polygonaceae plant species. To date, \u003ci\u003eChenopodium album WSCP\u003c/i\u003e (\u003ci\u003eCaWSCP\u003c/i\u003e) is the only cloned gene encoding a Class I WSCP. In this study, we identified two cDNAs encoding \u003ci\u003eChenopodium ficifolium\u003c/i\u003e Class I WSCPs, \u003ci\u003eCfWSCP1\u003c/i\u003e, and \u003ci\u003eCfWSCP2\u003c/i\u003e. Sequence analyses revealed that the open reading frames of \u003ci\u003eCfWSCP1\u003c/i\u003e and \u003ci\u003eCfWSCP2\u003c/i\u003e were 585 and 588 bp, respectively. Furthermore, both CfWSCPs contain cystein2 and cystein30, which are essential for the chlorophyll-binding ability of CaWSCP. Recombinant CfWSCP1 and CfWSCP2, expressed in \u003ci\u003eEscherichia coli\u003c/i\u003e as hexa-histidine fusion proteins (CfWSCP1-His and CfWSCP2-His), formed inclusion bodies; however, we were able to solubilize these using a buffer containing 8 M urea and then refold them by dialysis. The refolded CfWSCP1-His and CfWSCP2-His could bind chlorophylls and exhibited photoconvertibility, confirming that the cloned CfWSCPs are further examples of Class I WSCPs.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Identification_of_genes_encoding_photoconvertible_Class_I_water_soluble_chlorophyll_binding_proteins_from_i_Chenopodium_ficifolium_i_/1310466","contentUrl":null,"metadataVersion":7,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,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