{"data":{"id":"10.6084/m9.figshare.1306593.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1306593.v1","prefix":"10.6084","suffix":"m9.figshare.1306593.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Dean, Scott N.","nameType":"Personal","givenName":"Scott N.","familyName":"Dean","affiliation":[],"nameIdentifiers":[]},{"name":"Callee Walsh","affiliation":[],"nameIdentifiers":[]},{"name":"Haddon Goodman","affiliation":[],"nameIdentifiers":[]},{"name":"Hoek, Monique L. Van","nameType":"Personal","givenName":"Monique L. Van","familyName":"Hoek","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Analysis of mixed biofilm (\u003ci\u003eStaphylococcus aureus\u003c/i\u003e and \u003ci\u003ePseudomonas aeruginosa)\u003c/i\u003e by laser ablation electrospray ionization mass spectrometry"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"},{"subject":"Microbiology"}],"contributors":[],"dates":[{"date":"2015-02-07","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/08927014.2015.1011067","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1306593","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["806632 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003ePseudomonas aeruginosa\u003c/i\u003e and \u003ci\u003eStaphylococcus aureus\u003c/i\u003e are ubiquitous pathogens often found together in polymicrobial, biofilm-associated infections. This study is the first to use laser ablation electrospray ionization mass spectrometry (LAESI-MS) to rapidly study bacteria within a mixed biofilm. Fast, direct, non-invasive LAESI-MS analysis of biofilm could significantly accelerate biofilm studies and provide previously unavailable information on both biofilm composition and the effects of antibiofilm treatment. LAESI-MS was applied directly to a polymicrobial biofilm and analyzed with respect to whether \u003ci\u003eP. aeruginosa\u003c/i\u003e and \u003ci\u003eS. aureus\u003c/i\u003e were co-localized or self-segregated within the mixed biofilm. LAESI-MS was also used to analyze ions following LL-37 antimicrobial peptide treatment of the biofilm. This ambient ionization method holds promise for future biofilm studies. The use of this innovative technique has profound implications for the study of biofilms, as LAESI-MS eliminates the need for lengthy and disruptive sample preparation while permitting rapid analysis of unfixed and wet biofilms.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Analysis_of_mixed_biofilm_i_Staphylococcus_aureus_i_and_i_Pseudomonas_aeruginosa_i_by_laser_ablation_electrospray_ionization_mass_spectrometry/1306593/1","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"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