{"data":{"id":"10.6084/m9.figshare.1301099.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1301099.v1","prefix":"10.6084","suffix":"m9.figshare.1301099.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"He, Jun","givenName":"Jun","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"Yu, Jing-Jie","givenName":"Jing-Jie","familyName":"Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Xu, Qing","givenName":"Qing","familyName":"Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Lin","givenName":"Lin","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Liu, Ling-Zhi","givenName":"Ling-Zhi","familyName":"Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Jiang, Bing-Hua","givenName":"Bing-Hua","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]},{"name":"Z Zheng, Jenny","givenName":"Jenny","familyName":"Z Zheng","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Downregulation of ATG14 by EGR1-\u003ci\u003eMIR152\u003c/i\u003e sensitizes ovarian cancer cells to cisplatin-induced apoptosis by inhibiting cyto-protective autophagy"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Pharmacology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"}],"contributors":[],"dates":[{"date":"2015-02-01","dateType":"Created"},{"date":"2023-07-10","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1301099","relatedIdentifierType":"DOI"},{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15548627.2015.1009781","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1561779 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Cisplatin is commonly used in ovarian cancer treatment by inducing apoptosis in cancer cells as a result of lethal DNA damage. However, the intrinsic and acquired resistance to cisplatin in cancer cells remains a big challenge for improving overall survival. The cyto-protective functions of autophagy in cancer cells have been suggested as a potential mechanism for chemoresistance. Here, we reported \u003ci\u003eMIR152\u003c/i\u003e as a new autophagy-regulating miRNA that plays a role in cisplatin-resistance. We showed that \u003ci\u003eMIR152\u003c/i\u003e expression was dramatically downregulated in the cisplatin-resistant cell lines A2780/CP70, SKOV3/DDP compared with their respective parental cells, and in ovarian cancer tissues associated with cisplatin-resistance. Overexpression of \u003ci\u003eMIR152\u003c/i\u003e sensitized cisplatin-resistant ovarian cancer cells by reducing cisplatin-induced autophagy, enhancing cisplatin-induced apoptosis and inhibition of cell proliferation. A mouse subcutaneous xenograft tumor model using A2780/CP70 cells with overexpressing \u003ci\u003eMIR152\u003c/i\u003e was established and displayed decreased tumor growth in response to cisplatin. We also identified that ATG14 is a functional target of \u003ci\u003eMIR152\u003c/i\u003e in regulating autophagy inhibition. Furthermore, we found that EGR1 (early growth response 1) regulated the \u003ci\u003eMIR152\u003c/i\u003e gene at the transcriptional level. Ectopic expression of EGR1 enhanced efficacy of chemotherapy in A2780/CP70 cells. More importantly, these findings were relevant to clinical cases. Both EGR1 and \u003ci\u003eMIR152\u003c/i\u003e expression levels were significantly lower in ovarian cancer tissues with high levels of ERCC1 (excision repair cross-complementation group 1), a marker for cisplatin-resistance. Collectively, these data provide insights into novel mechanisms for acquired cisplatin-resistance. Activation of EGR1 and \u003ci\u003eMIR152\u003c/i\u003e may be a useful therapeutic strategy to overcome cisplatin-resistance by preventing cyto-protective autophagy in ovarian cancer.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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