{"data":{"id":"10.6084/m9.figshare.1296275.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1296275.v2","prefix":"10.6084","suffix":"m9.figshare.1296275.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wang, Zhenda","givenName":"Zhenda","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Hu, Jia","givenName":"Jia","familyName":"Hu","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Ge","givenName":"Ge","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Qu, Liujing","givenName":"Liujing","familyName":"Qu","affiliation":[],"nameIdentifiers":[]},{"name":"He, Qihua","givenName":"Qihua","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"Lou, Yaxin","givenName":"Yaxin","familyName":"Lou","affiliation":[],"nameIdentifiers":[]},{"name":"Song, Quansheng","givenName":"Quansheng","familyName":"Song","affiliation":[],"nameIdentifiers":[]},{"name":"Ma, Dalong","givenName":"Dalong","familyName":"Ma","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Yingyu","givenName":"Yingyu","familyName":"Chen","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Science Policy"},{"subject":"Cancer"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-06-09","dateType":"Created"},{"date":"2023-07-22","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1296275","relatedIdentifierType":"DOI"},{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/auto.36439","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["6082605 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Autophagy is a multistep process that involves the degradation and digestion of intracellular components by the lysosome. It has been proved that many core autophagy-related molecules participate in this event. However, new component proteins that regulate autophagy are still being discovered. At present, we report PHF23 (PHD finger protein 23) with a PHD-like zinc finger domain that can negatively regulate autophagy. Data from experiments indicated that the overexpression of PHF23 impaired autophagy, as characterized by decreased levels of LC3B-II and weakened degradation of endogenous and exogenous autophagic substrates. Conversely, knockdown of \u003ci\u003ePHF23\u003c/i\u003e resulted in opposite effects. Molecular mechanism studies suggested that PHF23 interacts with LRSAM1, which is an E3 ligase key for ubiquitin-dependent autophagy against invading bacteria. PHF23 promotes the ubiquitination and proteasome degradation of LRSAM1. We also show that the PHD finger of PHF23 is a functional domain needed for the interaction with LRSAM1. Altogether, our results indicate that PHF23 is a negative regulator associated in autophagy via the LRSAM1 signaling pathway. The physical and functional connection between the PHF23 and LRSAM1 needs further investigation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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