{"data":{"id":"10.6084/m9.figshare.1296275","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1296275","prefix":"10.6084","suffix":"m9.figshare.1296275","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wang, Zhenda","givenName":"Zhenda","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Hu, Jia","givenName":"Jia","familyName":"Hu","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Ge","givenName":"Ge","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Qu, Liujing","givenName":"Liujing","familyName":"Qu","affiliation":[],"nameIdentifiers":[]},{"name":"He, Qihua","givenName":"Qihua","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"Lou, Yaxin","givenName":"Yaxin","familyName":"Lou","affiliation":[],"nameIdentifiers":[]},{"name":"Song, Quansheng","givenName":"Quansheng","familyName":"Song","affiliation":[],"nameIdentifiers":[]},{"name":"Ma, Dalong","givenName":"Dalong","familyName":"Ma","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Yingyu","givenName":"Yingyu","familyName":"Chen","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Science Policy"}],"contributors":[],"dates":[{"date":"2015-06-09","dateType":"Created"},{"date":"2023-06-05","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/auto.36439","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["6082605 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Autophagy is a multistep process that involves the degradation and digestion of intracellular components by the lysosome. It has been proved that many core autophagy-related molecules participate in this event. However, new component proteins that regulate autophagy are still being discovered. At present, we report PHF23 (PHD finger protein 23) with a PHD-like zinc finger domain that can negatively regulate autophagy. Data from experiments indicated that the overexpression of PHF23 impaired autophagy, as characterized by decreased levels of LC3B-II and weakened degradation of endogenous and exogenous autophagic substrates. Conversely, knockdown of \u003ci\u003ePHF23\u003c/i\u003e resulted in opposite effects. Molecular mechanism studies suggested that PHF23 interacts with LRSAM1, which is an E3 ligase key for ubiquitin-dependent autophagy against invading bacteria. PHF23 promotes the ubiquitination and proteasome degradation of LRSAM1. We also show that the PHD finger of PHF23 is a functional domain needed for the interaction with LRSAM1. Altogether, our results indicate that PHF23 is a negative regulator associated in autophagy via the LRSAM1 signaling pathway. The physical and functional connection between the PHF23 and LRSAM1 needs further investigation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/PHF23_plant_homeodomain_finger_protein_23_negatively_regulates_cell_autophagy_by_promoting_ubiquitination_and_degradation_of_E3_ligase_LRSAM1/1296275/3","contentUrl":null,"metadataVersion":6,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":1,"viewsOverTime":[{"yearMonth":"2026-08","total":1}],"downloadCount":0,"downloadsOverTime":[{"yearMonth":"2026-08","total":0}],"referenceCount":0,"citationCount":1,"citationsOverTime":[{"year":"2024","total":1}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-01-28T20:48:43.000Z","registered":"2015-01-28T20:48:44.000Z","published":"2015","updated":"2024-03-24T11:48:33.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1296275","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.4161/auto.36439","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}