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But, how does a pure culture of the bacterium look like on solid agar medium? Answer to this question depends on the medium used. Typically, \u003ci\u003eE. coli\u003c/i\u003e DH5α would take on a beige, round colony morphology when grown on rich medium such as LB agar or Tryptic Soy Agar. However, the beige colour is absent from \u003ci\u003eE. coli\u003c/i\u003e DH5α colonies grown on minimal salts agar such as a formulated colourless agar used in this study. Pictures of \u003ci\u003eE. coli\u003c/i\u003e DH5α in pure culture on different agar media are encapsulated as a zip file dataset with this abstract. Incubation conditions were 37 \u003csup\u003eo\u003c/sup\u003eC under variable length of time on different agar media.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/Dataset_of_Escherichia_coli_DH5_in_pure_culture_on_different_agar_media/12948254/1","contentUrl":null,"metadataVersion":1,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":7,"viewsOverTime":[{"yearMonth":"2025-09","total":2},{"yearMonth":"2025-10","total":2},{"yearMonth":"2025-12","total":1},{"yearMonth":"2026-07","total":2}],"downloadCount":1,"downloadsOverTime":[{"yearMonth":"2025-09","total":1},{"yearMonth":"2025-10","total":0},{"yearMonth":"2025-12","total":0},{"yearMonth":"2026-07","total":0}],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2020-09-12T04:29:21.000Z","registered":"2020-09-12T04:29:21.000Z","published":"2020","updated":"2020-09-12T04:29:25.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.12948254.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}