{"data":{"id":"10.6084/m9.figshare.1292440.v6","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1292440.v6","prefix":"10.6084","suffix":"m9.figshare.1292440.v6","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Trojanowsky, Michelle","nameType":"Personal","givenName":"Michelle","familyName":"Trojanowsky","affiliation":[],"nameIdentifiers":[]},{"name":"Dusica Vidovic","affiliation":[],"nameIdentifiers":[]},{"name":"Simanski, Scott","nameType":"Personal","givenName":"Scott","familyName":"Simanski","affiliation":[],"nameIdentifiers":[]},{"name":"Penas, Clara","nameType":"Personal","givenName":"Clara","familyName":"Penas","affiliation":[],"nameIdentifiers":[]},{"name":"Schurer, Stephan","nameType":"Personal","givenName":"Stephan","familyName":"Schurer","affiliation":[],"nameIdentifiers":[]},{"name":"Ayad, Nagi G","nameType":"Personal","givenName":"Nagi G","familyName":"Ayad","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Screening of cell cycle fusion proteins to identify kinase signaling networks"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"Physiology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"80699 Information Systems not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Computer and information sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"60506 Virology","subjectScheme":"FOR"}],"contributors":[],"dates":[{"date":"2015-10-12","dateType":"Created"},{"date":"2019-04-02","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1006987","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1292440","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["254104 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Kinase signaling networks are well-established mediators of cell cycle transitions. However, how kinases interact with the ubiquitin proteasome system (UPS) to elicit protein turnover is not fully understood. We sought a means of identifying kinase-substrate interactions to better understand signaling pathways controlling protein degradation. Our prior studies used a luciferase fusion protein to uncover kinase networks controlling protein turnover. In this study, we utilized a similar approach to identify pathways controlling the cell cycle protein p27\u003csup\u003eKip1\u003c/sup\u003e. We generated a p27\u003csup\u003eKip1\u003c/sup\u003e-luciferase fusion and expressed it in cells incubated with compounds from a library of pharmacologically active compounds. We then compared the relative effects of the compounds on p27\u003csup\u003eKip1\u003c/sup\u003e-luciferase fusion stabilization. This was combined with \u003ci\u003ein silico\u003c/i\u003e kinome profiling to identify potential kinases inhibited by each compound. This approach effectively uncovered known kinases regulating p27\u003csup\u003eKip1\u003c/sup\u003e turnover. Collectively, our studies suggest that this parallel screening approach is robust and can be applied to fully understand kinase-ubiquitin pathway interactions.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjEyOTI0NDAuVjY8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5NaWNoZWxsZSBUcm9qYW5vd3NreTwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkR1c2ljYSBWaWRvdmljPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+U2NvdHQgU2ltYW5za2k8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5DbGFyYSBQZW5hczwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlN0ZXBoYW4gU2NodXJlcjwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPk5hZ2kgRyBBeWFkPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICA8L2NyZWF0b3JzPgogIDx0aXRsZXM+CiAgICA8dGl0bGU+U2NyZWVuaW5nIG9mIGNlbGwgY3ljbGUgZnVzaW9uIHByb3RlaW5zIHRvIGlkZW50aWZ5IGtpbmFzZSBzaWduYWxpbmcgbmV0d29ya3M8L3RpdGxlPgogIDwvdGl0bGVzPgogIDxkZXNjcmlwdGlvbnM+CiAgICA8ZGVzY3JpcHRpb24gZGVzY3JpcHRpb25UeXBlPSJBYnN0cmFjdCI+Jmx0O3AmZ3Q7S2luYXNlIHNpZ25hbGluZyBuZXR3b3JrcyBhcmUgd2VsbC1lc3RhYmxpc2hlZCBtZWRpYXRvcnMgb2YgY2VsbCBjeWNsZSB0cmFuc2l0aW9ucy4gSG93ZXZlciwgaG93IGtpbmFzZXMgaW50ZXJhY3Qgd2l0aCB0aGUgdWJpcXVpdGluIHByb3RlYXNvbWUgc3lzdGVtIChVUFMpIHRvIGVsaWNpdCBwcm90ZWluIHR1cm5vdmVyIGlzIG5vdCBmdWxseSB1bmRlcnN0b29kLiBXZSBzb3VnaHQgYSBtZWFucyBvZiBpZGVudGlmeWluZyBraW5hc2Utc3Vic3RyYXRlIGludGVyYWN0aW9ucyB0byBiZXR0ZXIgdW5kZXJzdGFuZCBzaWduYWxpbmcgcGF0aHdheXMgY29udHJvbGxpbmcgcHJvdGVpbiBkZWdyYWRhdGlvbi4gT3VyIHByaW9yIHN0dWRpZXMgdXNlZCBhIGx1Y2lmZXJhc2UgZnVzaW9uIHByb3RlaW4gdG8gdW5jb3ZlciBraW5hc2UgbmV0d29ya3MgY29udHJvbGxpbmcgcHJvdGVpbiB0dXJub3Zlci4gSW4gdGhpcyBzdHVkeSwgd2UgdXRpbGl6ZWQgYSBzaW1pbGFyIGFwcHJvYWNoIHRvIGlkZW50aWZ5IHBhdGh3YXlzIGNvbnRyb2xsaW5nIHRoZSBjZWxsIGN5Y2xlIHByb3RlaW4gcDI3Jmx0O3N1cCZndDtLaXAxJmx0Oy9zdXAmZ3Q7LiBXZSBnZW5lcmF0ZWQgYSBwMjcmbHQ7c3VwJmd0O0tpcDEmbHQ7L3N1cCZndDstbHVjaWZlcmFzZSBmdXNpb24gYW5kIGV4cHJlc3NlZCBpdCBpbiBjZWxscyBpbmN1YmF0ZWQgd2l0aCBjb21wb3VuZHMgZnJvbSBhIGxpYnJhcnkgb2YgcGhhcm1hY29sb2dpY2FsbHkgYWN0aXZlIGNvbXBvdW5kcy4gV2UgdGhlbiBjb21wYXJlZCB0aGUgcmVsYXRpdmUgZWZmZWN0cyBvZiB0aGUgY29tcG91bmRzIG9uIHAyNyZsdDtzdXAmZ3Q7S2lwMSZsdDsvc3VwJmd0Oy1sdWNpZmVyYXNlIGZ1c2lvbiBzdGFiaWxpemF0aW9uLiBUaGlzIHdhcyBjb21iaW5lZCB3aXRoICZsdDtpJmd0O2luIHNpbGljbyZsdDsvaSZndDsga2lub21lIHByb2ZpbGluZyB0byBpZGVudGlmeSBwb3RlbnRpYWwga2luYXNlcyBpbmhpYml0ZWQgYnkgZWFjaCBjb21wb3VuZC4gVGhpcyBhcHByb2FjaCBlZmZlY3RpdmVseSB1bmNvdmVyZWQga25vd24ga2luYXNlcyByZWd1bGF0aW5nIHAyNyZsdDtzdXAmZ3Q7S2lwMSZsdDsvc3VwJmd0OyB0dXJub3Zlci4gQ29sbGVjdGl2ZWx5LCBvdXIgc3R1ZGllcyBzdWdnZXN0IHRoYXQgdGhpcyBwYXJhbGxlbCBzY3JlZW5pbmcgYXBwcm9hY2ggaXMgcm9idXN0IGFuZCBjYW4gYmUgYXBwbGllZCB0byBmdWxseSB1bmRlcnN0YW5kIGtpbmFzZS11YmlxdWl0aW4gcGF0aHdheSBpbnRlcmFjdGlvbnMuJmx0Oy9wJmd0OzwvZGVzY3JpcHRpb24+CiAgPC9kZXNjcmlwdGlvbnM+CiAgPHN1YmplY3RzPgogICAgPHN1YmplY3Q+QmlvY2hlbWlzdHJ5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+TWVkaWNpbmU8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5NaWNyb2Jpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DZWxsIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5HZW5ldGljczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PlBoeXNpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzY2hlbWVVUkk9Imh0dHA6Ly93d3cuYWJzLmdvdi5hdS9hdXNzdGF0cy9hYnNALm5zZi8wLzZCQjQyN0FCOTY5NkMyMjVDQTI1NzQxODAwMDQ0NjNFIiBzdWJqZWN0U2NoZW1lPSJGT1IiPjM5OTk5IENoZW1pY2FsIFNjaWVuY2VzIG5vdCBlbHNld2hlcmUgY2xhc3NpZmllZDwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkltbXVub2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzY2hlbWVVUkk9Imh0dHA6Ly93d3cuYWJzLmdvdi5hdS9hdXNzdGF0cy9hYnNALm5zZi8wLzZCQjQyN0FCOTY5NkMyMjVDQTI1NzQxODAwMDQ0NjNFIiBzdWJqZWN0U2NoZW1lPSJGT1IiPjY5OTk5IEJpb2xvZ2ljYWwgU2NpZW5jZXMgbm90IGVsc2V3aGVyZSBjbGFzc2lmaWVkPC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc2NoZW1lVVJJPSJodHRwOi8vd3d3LmFicy5nb3YuYXUvYXVzc3RhdHMvYWJzQC5uc2YvMC82QkI0MjdBQjk2OTZDMjI1Q0EyNTc0MTgwMDA0NDYzRSIgc3ViamVjdFNjaGVtZT0iRk9SIj44MDY5OSBJbmZvcm1hdGlvbiBTeXN0ZW1zIG5vdCBlbHNld2hlcmUgY2xhc3NpZmllZDwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkRldmVsb3BtZW50YWwgQmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkNhbmNlcjwvc3ViamVjdD4KICAgIDxzdWJqZWN0IHNjaGVtZVVSST0iaHR0cDovL3d3dy5hYnMuZ292LmF1L2F1c3N0YXRzL2Fic0AubnNmLzAvNkJCNDI3QUI5Njk2QzIyNUNBMjU3NDE4MDAwNDQ2M0UiIHN1YmplY3RTY2hlbWU9IkZPUiI+MTEwMzA5IEluZmVjdGlvdXMgRGlzZWFzZXM8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5QbGFudCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc2NoZW1lVVJJPSJodHRwOi8vd3d3LmFicy5nb3YuYXUvYXVzc3RhdHMvYWJzQC5uc2YvMC82QkI0MjdBQjk2OTZDMjI1Q0EyNTc0MTgwMDA0NDYzRSIgc3ViamVjdFNjaGVtZT0iRk9SIj42MDUwNiBWaXJvbG9neTwvc3ViamVjdD4KICA8L3N1YmplY3RzPgogIDxwdWJsaXNoZXI+VGF5bG9yICZhbXA7IEZyYW5jaXM8L3B1Ymxpc2hlcj4KICA8cHVibGljYXRpb25ZZWFyPjIwMTU8L3B1YmxpY2F0aW9uWWVhcj4KICA8ZGF0ZXM+CiAgICA8ZGF0ZSBkYXRlVHlwZT0iQ3JlYXRlZCI+Mj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