{"data":{"id":"10.6084/m9.figshare.1292440.v4","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1292440.v4","prefix":"10.6084","suffix":"m9.figshare.1292440.v4","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Trojanowsky, Michelle","nameType":"Personal","givenName":"Michelle","familyName":"Trojanowsky","affiliation":[],"nameIdentifiers":[]},{"name":"Dusica Vidovic","affiliation":[],"nameIdentifiers":[]},{"name":"Simanski, Scott","nameType":"Personal","givenName":"Scott","familyName":"Simanski","affiliation":[],"nameIdentifiers":[]},{"name":"Penas, Clara","nameType":"Personal","givenName":"Clara","familyName":"Penas","affiliation":[],"nameIdentifiers":[]},{"name":"Schurer, Stephan","nameType":"Personal","givenName":"Stephan","familyName":"Schurer","affiliation":[],"nameIdentifiers":[]},{"name":"Ayad, Nagi G","nameType":"Personal","givenName":"Nagi G","familyName":"Ayad","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Screening of cell cycle fusion proteins to identify kinase signaling networks"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Developmental Biology"},{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Information and Computing Sciences"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Physiology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-09","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1292440","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["254104 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Kinase signaling networks are well-established mediators of cell cycle transitions. However, how kinases interact with the ubiquitin proteasome system (UPS) to elicit protein turnover is not fully understood. We sought a means of identifying kinase-substrate interactions to better understand signaling pathways controlling protein degradation. Our prior studies used a luciferase fusion protein to uncover kinase networks controlling protein turnover. In this study, we utilized a similar approach to identify pathways controlling the cell cycle protein p27\u003csup\u003eKip1\u003c/sup\u003e. We generated a p27\u003csup\u003eKip1\u003c/sup\u003e-luciferase fusion and expressed it in cells incubated with compounds from a library of pharmacologically active compounds. We then compared the relative effects of the compounds on p27\u003csup\u003eKip1\u003c/sup\u003e-luciferase fusion stabilization. This was combined with \u003ci\u003ein silico\u003c/i\u003e kinome profiling to identify potential kinases inhibited by each compound. This approach effectively uncovered known kinases regulating p27\u003csup\u003eKip1\u003c/sup\u003e turnover. Collectively, our studies suggest that this parallel screening approach is robust and can be applied to fully understand kinase-ubiquitin pathway interactions.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjEyOTI0NDAuVjQ8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5NaWNoZWxsZSBUcm9qYW5vd3NreTwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkR1c2ljYSBWaWRvdmljPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+U2NvdHQgU2ltYW5za2k8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5DbGFyYSBQZW5hczwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlN0ZXBoYW4gU2NodXJlcjwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPk5hZ2kgRyBBeWFkPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICA8L2NyZWF0b3JzPgogIDx0aXRsZXM+CiAgICA8dGl0bGU+U2NyZWVuaW5nIG9mIGNlbGwgY3ljbGUgZnVzaW9uIHByb3RlaW5zIHRvIGlkZW50aWZ5IGtpbmFzZSBzaWduYWxpbmcgbmV0d29ya3M8L3RpdGxlPgogIDwvdGl0bGVzPgogIDxkZXNjcmlwdGlvbnM+CiAgICA8ZGVzY3JpcHRpb24gZGVzY3JpcHRpb25UeXBlPSJBYnN0cmFjdCI+Jmx0O2RpdiZndDsmbHQ7cCZndDtLaW5hc2Ugc2lnbmFsaW5nIG5ldHdvcmtzIGFyZSB3ZWxsLWVzdGFibGlzaGVkIG1lZGlhdG9ycyBvZiBjZWxsIGN5Y2xlIHRyYW5zaXRpb25zLiBIb3dldmVyLCBob3cga2luYXNlcyBpbnRlcmFjdCB3aXRoIHRoZSB1YmlxdWl0aW4gcHJvdGVhc29tZSBzeXN0ZW0gKFVQUykgdG8gZWxpY2l0IHByb3RlaW4gdHVybm92ZXIgaXMgbm90IGZ1bGx5IHVuZGVyc3Rvb2QuIFdlIHNvdWdodCBhIG1lYW5zIG9mIGlkZW50aWZ5aW5nIGtpbmFzZS1zdWJzdHJhdGUgaW50ZXJhY3Rpb25zIHRvIGJldHRlciB1bmRlcnN0YW5kIHNpZ25hbGluZyBwYXRod2F5cyBjb250cm9sbGluZyBwcm90ZWluIGRlZ3JhZGF0aW9uLiBPdXIgcHJpb3Igc3R1ZGllcyB1c2VkIGEgbHVjaWZlcmFzZSBmdXNpb24gcHJvdGVpbiB0byB1bmNvdmVyIGtpbmFzZSBuZXR3b3JrcyBjb250cm9sbGluZyBwcm90ZWluIHR1cm5vdmVyLiBJbiB0aGlzIHN0dWR5LCB3ZSB1dGlsaXplZCBhIHNpbWlsYXIgYXBwcm9hY2ggdG8gaWRlbnRpZnkgcGF0aHdheXMgY29udHJvbGxpbmcgdGhlIGNlbGwgY3ljbGUgcHJvdGVpbiBwMjcmbHQ7c3VwJmd0O0tpcDEmbHQ7L3N1cCZndDsuIFdlIGdlbmVyYXRlZCBhIHAyNyZsdDtzdXAmZ3Q7S2lwMSZsdDsvc3VwJmd0Oy1sdWNpZmVyYXNlIGZ1c2lvbiBhbmQgZXhwcmVzc2VkIGl0IGluIGNlbGxzIGluY3ViYXRlZCB3aXRoIGNvbXBvdW5kcyBmcm9tIGEgbGlicmFyeSBvZiBwaGFybWFjb2xvZ2ljYWxseSBhY3RpdmUgY29tcG91bmRzLiBXZSB0aGVuIGNvbXBhcmVkIHRoZSByZWxhdGl2ZSBlZmZlY3RzIG9mIHRoZSBjb21wb3VuZHMgb24gcDI3Jmx0O3N1cCZndDtLaXAxJmx0Oy9zdXAmZ3Q7LWx1Y2lmZXJhc2UgZnVzaW9uIHN0YWJpbGl6YXRpb24uIFRoaXMgd2FzIGNvbWJpbmVkIHdpdGggJmx0O2kmZ3Q7aW4gc2lsaWNvJmx0Oy9pJmd0OyBraW5vbWUgcHJvZmlsaW5nIHRvIGlkZW50aWZ5IHBvdGVudGlhbCBraW5hc2VzIGluaGliaXRlZCBieSBlYWNoIGNvbXBvdW5kLiBUaGlzIGFwcHJvYWNoIGVmZmVjdGl2ZWx5IHVuY292ZXJlZCBrbm93biBraW5hc2VzIHJlZ3VsYXRpbmcgcDI3Jmx0O3N1cCZndDtLaXAxJmx0Oy9zdXAmZ3Q7IHR1cm5vdmVyLiBDb2xsZWN0aXZlbHksIG91ciBzdHVkaWVzIHN1Z2dlc3QgdGhhdCB0aGlzIHBhcmFsbGVsIHNjcmVlbmluZyBhcHByb2FjaCBpcyByb2J1c3QgYW5kIGNhbiBiZSBhcHBsaWVkIHRvIGZ1bGx5IHVuZGVyc3RhbmQga2luYXNlLXViaXF1aXRpbiBwYXRod2F5IGludGVyYWN0aW9ucy4mbHQ7L3AmZ3Q7Jmx0Oy9kaXYmZ3Q7PC9kZXNjcmlwdGlvbj4KICA8L2Rlc2NyaXB0aW9ucz4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdD5EZXZlbG9wbWVudGFsIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzY2hlbWVVUkk9Imh0dHA6Ly93d3cuYWJzLmdvdi5hdS9hdXNzdGF0cy9hYnNALm5zZi8wLzZCQjQyN0FCOTY5NkMyMjVDQTI1NzQxODAwMDQ0NjNFIiBzdWJqZWN0U2NoZW1lPSJGT1IiPjYwNTA2IFZpcm9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+UGxhbnQgQmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0IHNjaGVtZVVSST0iaHR0cDovL3d3dy5hYnMuZ292LmF1L2F1c3N0YXRzL2Fic0AubnNmLzAvNkJCNDI3QUI5Njk2QzIyNUNBMjU3NDE4MDAwNDQ2M0UiIHN1YmplY3RTY2hlbWU9IkZPUiI+MTEwMzA5IEluZmVjdGlvdXMgRGlzZWFzZXM8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5JbmZvcm1hdGlvbiBhbmQgQ29tcHV0aW5nIFNjaWVuY2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvbG9naWNhbCBTY2llbmNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkltbXVub2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5QaHlzaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+R2VuZXRpY3M8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DZWxsIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5NaWNyb2Jpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9jaGVtaXN0cnk8L3N1YmplY3Q+CiAgPC9zdWJqZWN0cz4KICA8cHVibGlzaGVyPlRheWxvciAmYW1wOyBGcmFuY2lzPC9wdWJsaXNoZXI+CiAgPHB1YmxpY2F0aW9uWWVhcj4yMDE1PC9wdWJsaWNhdGlvblllYXI+CiAgPGRhdGVzPgogICAgPGRhdGUgZGF0ZVR5cGU9IkNyZWF0ZWQiPjIwMTUtMTAtMDk8L2RhdGU+CiAgICA8ZGF0ZSBkYXRlVHlwZT0iVXBkYXRlZCI+MjAxNi0wMS0xOTwvZGF0ZT4KICA8L2RhdGVzPgogIDxyZXNvdXJjZVR5cGUgcmVzb3VyY2VUeXBlR2VuZXJhbD0iRGF0YXNldCI+RGF0YXNldDwvcmVzb3VyY2VUeXBlPgogIDxzaXplcz4KICAgIDxzaXplPjI1NDEwNCBCeXRlczwvc2l6ZT4KICA8L3NpemVzPgogIDxyZWxhdGVkSWRlbnRpZmllcnM+CiAgICA8cmVsYXRlZElkZW50aWZpZXIgcmVsYXRlZElkZW50aWZpZXJUeXBlPSJET0kiIHJlbGF0aW9uVHlwZT0iSXNQcmV2aW91c1ZlcnNpb25PZiI+MTAuNjA4NC9tOS5maWdzaGFyZS4xMjkyNDQwPC9yZWxhdGVkSWRlbnRpZmllcj4KICA8L3JlbGF0ZWRJZGVudGlmaWVycz4KICA8cmlnaHRzTGlzdD4KICAgIDxyaWdodHMgcmlnaHRzVVJJPSJodHRwczovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW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