{"data":{"id":"10.6084/m9.figshare.12909863","type":"dois","attributes":{"doi":"10.6084/m9.figshare.12909863","prefix":"10.6084","suffix":"m9.figshare.12909863","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Ningyong Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Ayers, Linn","nameType":"Personal","givenName":"Linn","familyName":"Ayers","affiliation":[],"nameIdentifiers":[]},{"name":"Pastukh, Viktoriya","nameType":"Personal","givenName":"Viktoriya","familyName":"Pastukh","affiliation":[],"nameIdentifiers":[]},{"name":"Alexeyev, Mikhail","nameType":"Personal","givenName":"Mikhail","familyName":"Alexeyev","affiliation":[],"nameIdentifiers":[]},{"name":"Stevens, Troy","nameType":"Personal","givenName":"Troy","familyName":"Stevens","affiliation":[],"nameIdentifiers":[]},{"name":"Dhananjay T. Tambe","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Supplementary material - Impact of Na+ permeation on collective migration of pulmonary arterial endothelial cells (Xu et al)"}],"publisher":"figshare","container":{},"publicationYear":2020,"subjects":[{"subject":"111601 Cell Physiology","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2020-09-02","dateType":"Created"},{"date":"2020-10-19","dateType":"Updated"},{"date":"2020","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["73593086 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003cb\u003eEndothelial migration is necessary for wound healing and angiogenesis. Na\u003csup\u003e+\u003c/sup\u003e has been incriminated as an important cellular signal mediating migration, although the ion channels regulating Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e permeation that contribute to migration remain poorly understood. First, we studied the contribution of extracellular Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e to scratch wound healing. Second, we studied the effects of Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e channel inhibitors inhibiting ENaC, NCX, Orai1, and/or TRPC4 to scratch wound healing. Third, we studied the contribution of Orai1 expression to scratch and non-scratch wound healing in the presence and absence of extracellular Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e. All studies were conducted using pulmonary artery endothelial cells (PAEC). We found that permeation of extracellular Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e is necessary for efficient and cohesive migration of PAEC monolayers. The processes of wound healing displayed two phases: a fast healing phase within 5 hours followed by a slow-healing phase between 5 and 24 hours. Among the examined Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e channels, Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e permeation through ENaC produced prompt and largest effect on wound healing, Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e permeation through NCX produced small and delayed effect, Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+ \u003c/sup\u003e\u003c/b\u003epermeation through Orai1 produced small and short-term effect, and Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+ \u003c/sup\u003e\u003c/b\u003epermeation through TRPC1/4 produced insignificant effect. Both extracellular Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e and Orai1 contribute to scratch wound healing, whereas only extracellular Na\u003c/b\u003e\u003cb\u003e\u003cb\u003e\u003csup\u003e+\u003c/sup\u003e\u003c/b\u003e but not Orai1 contributes to cell migration in the absence of injury. \u003cbr\u003e\u003c/b\u003e","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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