{"data":{"id":"10.6084/m9.figshare.1289848.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1289848.v3","prefix":"10.6084","suffix":"m9.figshare.1289848.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Kozakova, Lucie","nameType":"Personal","givenName":"Lucie","familyName":"Kozakova","affiliation":[],"nameIdentifiers":[]},{"name":"Vondrova, Lucie","nameType":"Personal","givenName":"Lucie","familyName":"Vondrova","affiliation":[],"nameIdentifiers":[]},{"name":"Stejskal, Karel","nameType":"Personal","givenName":"Karel","familyName":"Stejskal","affiliation":[],"nameIdentifiers":[]},{"name":"Charalabous, Panagoula","nameType":"Personal","givenName":"Panagoula","familyName":"Charalabous","affiliation":[],"nameIdentifiers":[]},{"name":"Kolesar, Peter","nameType":"Personal","givenName":"Peter","familyName":"Kolesar","affiliation":[],"nameIdentifiers":[]},{"name":"Lehmann, Alan R","nameType":"Personal","givenName":"Alan R","familyName":"Lehmann","affiliation":[],"nameIdentifiers":[]},{"name":"Uldrijan, Stjepan","nameType":"Personal","givenName":"Stjepan","familyName":"Uldrijan","affiliation":[],"nameIdentifiers":[]},{"name":"Sanderson, Christopher M","nameType":"Personal","givenName":"Christopher M","familyName":"Sanderson","affiliation":[],"nameIdentifiers":[]},{"name":"Zbynek Zdrahal","affiliation":[],"nameIdentifiers":[]},{"name":"Palecek, Jan J","nameType":"Personal","givenName":"Jan J","familyName":"Palecek","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Evolutionary Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-09","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2014.1000112","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1289848","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["43582314 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The MAGE (Melanoma-associated antigen) protein family members are structurally related to each other by a MAGE-homology domain comprised of 2 winged helix motifs WH/A and WH/B. This family specifically evolved in placental mammals although single homologs designated NSE3 (non-SMC element) exist in most eukaryotes. NSE3, together with its partner proteins NSE1 and NSE4 form a tight subcomplex of the structural maintenance of chromosomes SMC5–6 complex. Previously, we showed that interactions of the WH/B motif of the MAGE proteins with their NSE4/EID partners are evolutionarily conserved (including the MAGEA1-NSE4 interaction). In contrast, the interaction of the WH/A motif of NSE3 with NSE1 diverged in the MAGE paralogs. We hypothesized that the MAGE paralogs acquired new RING-finger-containing partners through their evolution and form MAGE complexes reminiscent of NSE1-NSE3-NSE4 trimers. In this work, we employed the yeast 2-hybrid system to screen a human RING-finger protein library against several MAGE baits. We identified a number of potential MAGE-RING interactions and confirmed several of them (MDM4, PCGF6, RNF166, TRAF6, TRIM8, TRIM31, TRIM41) in co-immunoprecipitation experiments. Among these MAGE-RING pairs, we chose to examine MAGEA1-TRIM31 in detail and showed that both WH/A and WH/B motifs of MAGEA1 bind to the coiled-coil domain of TRIM31 and that MAGEA1 interaction stimulates TRIM31 ubiquitin-ligase activity. In addition, TRIM31 directly binds to NSE4, suggesting the existence of a TRIM31-MAGEA1-NSE4 complex reminiscent of the NSE1-NSE3-NSE4 trimer. These results suggest that MAGEA1 functions as a co-factor of TRIM31 ubiquitin-ligase and that the TRIM31-MAGEA1-NSE4 complex may have evolved from an ancestral NSE1-NSE3-NSE4 complex.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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