{"data":{"id":"10.6084/m9.figshare.1284401","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1284401","prefix":"10.6084","suffix":"m9.figshare.1284401","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jin, Lin","nameType":"Personal","givenName":"Lin","familyName":"Jin","affiliation":[],"nameIdentifiers":[]},{"name":"Pran K Datta","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Oncogenic STRAP functions as a novel negative regulator of E-cadherin and p21\u003csup\u003eCip1\u003c/sup\u003e by modulating the transcription factor Sp1"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Biotechnology"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2015-01-04","dateType":"Created"},{"date":"2020-05-18","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15384101.2014.973310","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["570118 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"We have previously reported the identification of a novel WD-domain protein, STRAP that plays a role in maintenance of mesenchymal morphology by regulating E-cadherin and that enhances tumorigenicity partly by downregulating CDK inhibitor p21\u003csup\u003eCip1\u003c/sup\u003e. However, the functional mechanism of regulation of E-cadherin and p21\u003csup\u003eCip1\u003c/sup\u003e by STRAP is unknown. Here, we have employed STRAP knock out and knockdown cell models (mouse embryonic fibroblast, human cancer cell lines) to show how STRAP downregulates E-cadherin and p21\u003csup\u003eCip1\u003c/sup\u003e by abrogating the binding of Sp1 to its consensus binding sites. Moreover, ChIP assays suggest that STRAP recruits HDAC1 to Sp1 binding sites in p21\u003csup\u003eCip1\u003c/sup\u003e promoter. Interestingly, loss of STRAP can stabilize Sp1 by repressing its ubiquitination in G1 phase, resulting in an enhanced expression of p21\u003csup\u003eCip1\u003c/sup\u003e by \u0026gt;4.5-fold and cell cycle arrest. Using Bioinformatics and Microarray analyses, we have observed that 87% mouse genes downregulated by STRAP have conserved Sp1 binding sites. In NSCLC, the expression levels of STRAP inversely correlated with that of Sp1 (60%). These results suggest a novel mechanism of regulation of E-cadherin and p21\u003csup\u003eCip1\u003c/sup\u003e by STRAP by modulating Sp1-dependent transcription, and higher expression of STRAP in lung cancer may contribute to downregulation of E-cadherin and p21\u003csup\u003eCip1\u003c/sup\u003e and to tumor progression.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Oncogenic_STRAP_functions_as_a_novel_negative_regulator_of_E_cadherin_and_p21_sup_Cip1_sup_by_modulating_the_transcription_factor_Sp1_/1284401","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://data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