{"data":{"id":"10.6084/m9.figshare.1282587.v4","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1282587.v4","prefix":"10.6084","suffix":"m9.figshare.1282587.v4","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Miwa, Shinji","nameType":"Personal","givenName":"Shinji","familyName":"Miwa","affiliation":[],"nameIdentifiers":[]},{"name":"Shuya Yano","affiliation":[],"nameIdentifiers":[]},{"name":"Kimura, Hiroaki","nameType":"Personal","givenName":"Hiroaki","familyName":"Kimura","affiliation":[],"nameIdentifiers":[]},{"name":"Yamamoto, Mako","nameType":"Personal","givenName":"Mako","familyName":"Yamamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Toneri, Makoto","nameType":"Personal","givenName":"Makoto","familyName":"Toneri","affiliation":[],"nameIdentifiers":[]},{"name":"Matsumoto, Yasunori","nameType":"Personal","givenName":"Yasunori","familyName":"Matsumoto","affiliation":[],"nameIdentifiers":[]},{"name":"Fuminari Uehara","affiliation":[],"nameIdentifiers":[]},{"name":"Hiroshima, Yukihiko","nameType":"Personal","givenName":"Yukihiko","familyName":"Hiroshima","affiliation":[],"nameIdentifiers":[]},{"name":"Murakami, Takashi","nameType":"Personal","givenName":"Takashi","familyName":"Murakami","affiliation":[],"nameIdentifiers":[]},{"name":"Hayashi, Katsuhiro","nameType":"Personal","givenName":"Katsuhiro","familyName":"Hayashi","affiliation":[],"nameIdentifiers":[]},{"name":"Yamamoto, Norio","nameType":"Personal","givenName":"Norio","familyName":"Yamamoto","affiliation":[],"nameIdentifiers":[]},{"name":"Bouvet, Michael","nameType":"Personal","givenName":"Michael","familyName":"Bouvet","affiliation":[],"nameIdentifiers":[]},{"name":"Toshiyoshi Fujiwara","affiliation":[],"nameIdentifiers":[]},{"name":"Tsuchiya, Hiroyuki","nameType":"Personal","givenName":"Hiroyuki","familyName":"Tsuchiya","affiliation":[],"nameIdentifiers":[]},{"name":"Hoffman, Robert M","nameType":"Personal","givenName":"Robert M","familyName":"Hoffman","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Cell-cycle fate-monitoring distinguishes individual chemosensitive and chemoresistant cancer cells in drug-treated heterogeneous populations demonstrated by real-time FUCCI imaging"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cell Biology"},{"subject":"Pharmacology"},{"subject":"Biotechnology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2015-10-09","dateType":"Created"},{"date":"2020-06-17","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15384101.2014.991604","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1282587","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["22185651 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Essentially every population of cancer cells within a tumor is heterogeneous, especially with regard to chemosensitivity and resistance. In the present study, we utilized the fluorescence ubiquitination-based cell cycle indicator (FUCCI) imaging system to investigate the correlation between cell-cycle behavior and apoptosis after treatment of cancer cells with chemotherapeutic drugs. HeLa cells expressing FUCCI were treated with doxorubicin (DOX) (5 μM) or cisplatinum (CDDP) (5 μM) for 3 h. Cell-cycle progression and apoptosis were monitored by time-lapse FUCCI imaging for 72 h. Time-lapse FUCCI imaging demonstrated that both DOX and CDDP could induce cell cycle arrest in S/G\u003csub\u003e2\u003c/sub\u003e/M in almost all the cells, but a subpopulation of the cells could escape the block and undergo mitosis. The subpopulation which went through mitosis subsequently underwent apoptosis, while the cells arrested in S/G\u003csub\u003e2\u003c/sub\u003e/M survived. The present results demonstrate that chemoresistant cells can be readily identified in a heterogeneous population of cancer cells by S/G\u003csub\u003e2\u003c/sub\u003e/M arrest, which can serve in future studies as a visible target for novel agents that kill cell-cycle-arrested cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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