{"data":{"id":"10.6084/m9.figshare.1276010.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1276010.v2","prefix":"10.6084","suffix":"m9.figshare.1276010.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Pant, Shankar R","nameType":"Personal","givenName":"Shankar R","familyName":"Pant","affiliation":[],"nameIdentifiers":[]},{"name":"Krishnavajhala, Aparna","nameType":"Personal","givenName":"Aparna","familyName":"Krishnavajhala","affiliation":[],"nameIdentifiers":[]},{"name":"McNeece, Brant T","nameType":"Personal","givenName":"Brant T","familyName":"McNeece","affiliation":[],"nameIdentifiers":[]},{"name":"Lawrence, Gary W","nameType":"Personal","givenName":"Gary W","familyName":"Lawrence","affiliation":[],"nameIdentifiers":[]},{"name":"Klink, Vincent P","nameType":"Personal","givenName":"Vincent P","familyName":"Klink","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The syntaxin 31-induced gene, LESION SIMULATING DISEASE1 (LSD1), functions in \u003ci\u003eGlycine max\u003c/i\u003e defense to the root parasite \u003ci\u003eHeterodera glycines\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Molecular Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-21","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15592324.2014.977737","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1276010","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3141373 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Experiments show the membrane fusion genes α soluble NSF attachment protein (α-SNAP) and syntaxin 31 (Gm-SYP38) contribute to the ability of \u003ci\u003eGlycine max\u003c/i\u003e to defend itself from infection by the plant parasitic nematode \u003ci\u003eHeterodera glycines\u003c/i\u003e. Accompanying their expression is the transcriptional activation of the defense genes ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) and NONEXPRESSOR OF PR1 (NPR1) that function in salicylic acid (SA) signaling. These results implicate the added involvement of the antiapoptotic, environmental response gene LESION SIMULATING DISEASE1 (LSD1) in defense. Roots engineered to overexpress the \u003ci\u003eG. max\u003c/i\u003e defense genes Gm-α-SNAP, SYP38, EDS1, NPR1, BOTRYTIS INDUCED KINASE1 (BIK1) and xyloglucan endotransglycosylase/hydrolase (XTH) in the susceptible genotype \u003ci\u003eG. max\u003c/i\u003e\u003csub\u003e[Williams 82/PI 518671]\u003c/sub\u003e have induced Gm-LSD1 (Gm-LSD1–2) transcriptional activity. In reciprocal experiments, roots engineered to overexpress Gm-LSD1–2 in the susceptible genotype \u003ci\u003eG. max\u003c/i\u003e\u003csub\u003e[Williams 82/PI 518671]\u003c/sub\u003e have induced levels of SYP38, EDS1, NPR1, BIK1 and XTH, but not α-SNAP prior to infection. In tests examining the role of Gm-LSD1–2 in defense, its overexpression results in ∼52 to 68% reduction in nematode parasitism. In contrast, RNA interference (RNAi) of Gm-LSD1–2 in the resistant genotype \u003ci\u003eG. max\u003c/i\u003e\u003csub\u003e[Peking/PI 548402]\u003c/sub\u003e results in an 3.24–10.42 fold increased ability of \u003ci\u003eH. glycines\u003c/i\u003e to parasitize. The results identify that Gm-LSD1–2 functions in the defense response of \u003ci\u003eG. max\u003c/i\u003e to \u003ci\u003eH. glycines\u003c/i\u003e parasitism. It is proposed that LSD1, as an antiapoptotic protein, may establish an environment whereby the protected, living plant cell could secrete materials in the vicinity of the parasitizing nematode to disarm it. After the targeted incapacitation of the nematode the parasitized cell succumbs to its targeted demise as the infected root region is becoming fortified.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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