{"data":{"id":"10.6084/m9.figshare.1275099.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1275099.v2","prefix":"10.6084","suffix":"m9.figshare.1275099.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Li, Feng-Jun","givenName":"Feng-Jun","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Y He, Cynthia","givenName":"Cynthia","familyName":"Y He","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Acidocalcisome is required for autophagy in \u003ci\u003eTrypanosoma brucei\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Physics"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-02-27","dateType":"Created"},{"date":"2026-02-17","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1275099","relatedIdentifierType":"DOI"},{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/auto.36183","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["9717830 Bytes"],"formats":[],"version":"2","rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Lysosomes play important roles in autophagy, not only in autophagosome degradation, but also in autophagy initiation. In \u003ci\u003eTrypanosoma brucei,\u003c/i\u003e an early divergent protozoan parasite\u003ci\u003e,\u003c/i\u003e we discovered a previously unappreciated function of the acidocalcisome, a lysosome-related organelle characterized by acidic pH and large content of Ca\u003csup\u003e2+\u003c/sup\u003e and polyphosphates, in autophagy regulation. Starvation- and chemical-induced autophagy is accompanied with acidocalcisome acidification, and blocking the acidification completely inhibits autophagosome formation. Blocking acidocalcisome biogenesis by depleting the adaptor protein-3 complex, which does not affect lysosome biogenesis or function, also inhibits autophagy. Overall, our results support the role of the acidocalcisome, a conserved organelle from bacteria to human, as a relevant regulator in autophagy.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Acidocalcisome_is_required_for_autophagy_in_i_Trypanosoma_brucei_i_/1275099/2","contentUrl":null,"metadataVersion":5,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":1,"citationsOverTime":[{"year":"2026","total":1}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T12:27:49.000Z","registered":"2016-01-19T12:27:50.000Z","published":"2015","updated":"2026-02-17T01:23:56.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1275099.v2","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.4161/auto.36183","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"version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