{"data":{"id":"10.6084/m9.figshare.1275093","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1275093","prefix":"10.6084","suffix":"m9.figshare.1275093","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Gu, Wenwen","givenName":"Wenwen","familyName":"Gu","affiliation":[],"nameIdentifiers":[]},{"name":"Yang, Long","givenName":"Long","familyName":"Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Shuangjie","givenName":"Shuangjie","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Jiang, Lihua","givenName":"Lihua","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Generation and Application of a Novel InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e Mono-Antibody from Mouse"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Cardiology"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Biotechnology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Neuroscience"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-03-20","dateType":"Created"},{"date":"2023-06-01","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15321819.2014.996817","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["5796369 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Inositol 1, 4, 5-Trisphosphate Receptor (InsP\u003csub\u003e3\u003c/sub\u003eR) is an intracellular Ca\u003csup\u003e2+\u003c/sup\u003e release channel, which widely participates in cellular processes. Three isoforms of InsP\u003csub\u003e3\u003c/sub\u003eR were identified as InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e, InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e2,\u003c/sub\u003e and InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e3\u003c/sub\u003e. They share 60–0% protein sequence homology and form a channel in a manner of homotetramer or heterotetramer. Several InsP\u003csub\u003e3\u003c/sub\u003eR isoform-specific rabbit antibodies have been generated to distinguish their localization and functions. However, there is no report of such a valid antibody raised from other species. In his article, we prepare a mouse monoclonal antibody against a synthetic peptide with rat InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e-specific carboxyl terminus sequence. This monoclonal antibody of InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e (R1-mAb) was purified and characterized as IgG2b, which can recognize InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e by Western-blot (WB) analysis and immunoprecipitate (IP) InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e from moue brain lysate tested. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody could give the consistent results in SH-SY5Y cells, human sperm, and mouse brain paraffin section. In summary, we generate a mouse InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e-specific IgG 2b antibody identifying InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e in WB, IF, IHC, and IP analysis, which provides a possible choice for detection of InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e, especially in application of co-localization analysis with other InsP\u003csub\u003e3\u003c/sub\u003eR isoforms or other 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