{"data":{"id":"10.6084/m9.figshare.1267559.v3","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1267559.v3","prefix":"10.6084","suffix":"m9.figshare.1267559.v3","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Tak, Eun Sik","nameType":"Personal","givenName":"Eun Sik","familyName":"Tak","affiliation":[],"nameIdentifiers":[]},{"name":"Cho, Sung-Jin","nameType":"Personal","givenName":"Sung-Jin","familyName":"Cho","affiliation":[],"nameIdentifiers":[]},{"name":"Park, Soon Cheol","nameType":"Personal","givenName":"Soon Cheol","familyName":"Park","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Gene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, \u003ci\u003eEisenia andrei\u003c/i\u003e, using expressed sequence tags"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Evolutionary Biology"},{"subject":"Physiology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-03-09","dateType":"Created"},{"date":"2016-01-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2014.988677","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1267559","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["134900 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions. To gain insight into the expression profiles of coelomic cells of the earthworm, \u003ci\u003eEisenia andrei\u003c/i\u003e, we analyzed 1151 expressed sequence tags (ESTs) derived from the cDNA library of the coelomic cells. Among the 1151 ESTs analyzed, 493 ESTs (42.8%) showed a significant similarity to known genes and represented 164 unique genes, of which 93 ESTs were singletons and 71 ESTs manifested as two or more ESTs. From the 164 unique genes sequenced, we found 24 immune-related and cell defense genes. Furthermore, real-time PCR analysis showed that levels of lysenin-related proteins mRNA in coelomic cells of \u003ci\u003eE. andrei\u003c/i\u003e were upregulated after the injection of \u003ci\u003eBacillus subtilis\u003c/i\u003e bacteria. This EST data-set would provide a valuable resource for future researches of earthworm immune system.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Gene_expression_profiling_of_coelomic_cells_and_discovery_of_immune_related_genes_in_the_earthworm_i_Eisenia_andrei_i_using_expressed_sequence_tags/1267559/3","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-19T12:08:41.000Z","registered":"2016-01-19T12:08:42.000Z","published":"2015","updated":"2020-09-04T20:02:32.000Z"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